Di Virgilio A L, Arnal P M, Maisuls I
CEQUINOR (Centro de Química Inorgánica), Facultad de Ciencias Exactas, UNLP. 47 y 115 (1900), La Plata, Argentina; Cátedra de Bioquímica Patológica, Facultad de Ciencias Exactas, UNLP. 47 y 115 (1900), La Plata, Argentina.
CETMIC (Centro de Tecnología de Recursos Minerales y Cerámica), Cno Centenario y 506, CC 49 (B1897ZCA), M.B. Gonnet, Prov. Buenos Aires, Argentina.
Mutat Res Genet Toxicol Environ Mutagen. 2014 Aug;770:85-94. doi: 10.1016/j.mrgentox.2014.05.009. Epub 2014 Jun 6.
The cytotoxicity and genotoxicity of novel colloidal silica spheres coated with crystalline or amorphous zirconia (SiO2@ZrO2(cryst) or SiO2@ZrO2(am)) have been studied in a human osteosarcoma cell line (MG-63), after 24 h exposure. SiO2@ZrO2(cryst) and SiO2@ZrO2(am) had mean diameters of 782±19 and 891±34 nm, respectively. SiO2@ZrO2(cryst) exposure reduced cell viability, with an increase in reactive oxygen species (ROS) and a decrease of the GSH/GSSG ratio. The comet and micronucleus (MN) assays detected DNA damage at 5 and 25 μg/mL, respectively. SiO2@ZrO2(am) induced genotoxic action only at 10 and 50 μg/mL (comet and MN assays), along with a decrease of the GSH/GSSG ratio at 50 μg/mL. Both particles were found inside the cells, forming vesicles; however, none of them entered the nucleus. Our findings show that crystallization of the shell of the amorphous ZrO2 increases both cytotoxicity and genotoxicity.
在暴露24小时后，已对涂覆有结晶或无定形氧化锆的新型胶体二氧化硅球（SiO2@ZrO2(cryst)或SiO2@ZrO2(am)）在人骨肉瘤细胞系（MG-63）中的细胞毒性和遗传毒性进行了研究。SiO2@ZrO2(cryst)和SiO2@ZrO2(am)的平均直径分别为782±19和891±34纳米。暴露于SiO2@ZrO2(cryst)会降低细胞活力，活性氧（ROS）增加，谷胱甘肽/氧化型谷胱甘肽（GSH/GSSG）比值降低。彗星试验和微核（MN）试验分别在5和25微克/毫升时检测到DNA损伤。SiO2@ZrO2(am)仅在10和50微克/毫升时诱导遗传毒性作用（彗星试验和MN试验），并且在50微克/毫升时GSH/GSSG比值降低。两种颗粒都在细胞内被发现，形成囊泡；然而，它们都没有进入细胞核。我们的研究结果表明，无定形ZrO2壳层的结晶增加了细胞毒性和遗传毒性。
