Li Mingying, Zhang Xuena, Wu Anshi, Wang Zhenyuan, Li Jie, Yue Yun
Department of Anaesthesiology, Beijing Chaoyang Hospital, Capital Medical University, Beijing, 100020, China.
Cell Biochem Biophys. 2015 Mar;71(2):1059-66. doi: 10.1007/s12013-014-0309-6.
Elderly patients may experience a decline in cognition after a surgery performed under anesthesia. Propofol (2,6-diisopropylphenol), a common intravenous anesthetic agent, has been reported to mediate the long-term potentiation (LTP), a major form of synaptic plasticity. The present study was conducted to investigate the underlying mechanisms in young (3-month-old) and elderly (20-month-old) male rats. A decline of theta-burst stimulation (TBS)-induced LTP in the hippocampal CA1 area was found in the young rats at 72 h post-anesthesia, and this alteration almost disappeared after 2-week-recovery as compared with their age-matched control rats. On the other hand, the propofol-induced CA1 LTP reduction was persistent in the aged rats during the whole experimental process. Moreover, TBS-induced increases in CA 1 filamentous-actin (F-actin) polymerization and phospho-cofilin expression were enhanced at 72 h post-anesthesia in young rats, and this change was significantly attenuated after 2 weeks. However, in anesthetic elderly rats, the alterations in F-actin and phospho-cofilin of the CA1 region were still presented at the end of the experiments. Taken together, our results indicate that the discrepant responses between young and aged rats to propofol anesthesia may be associated with the differential polymerization of F-actin.
老年患者在接受麻醉手术后可能会出现认知能力下降。丙泊酚（2,6-二异丙基苯酚）是一种常用的静脉麻醉剂，据报道它能介导长时程增强（LTP），这是突触可塑性的一种主要形式。本研究旨在探究年轻（3个月大）和老年（20个月大）雄性大鼠的潜在机制。在麻醉后72小时，年轻大鼠海马CA1区theta爆发刺激（TBS）诱导的LTP出现下降，与年龄匹配的对照大鼠相比，这种改变在2周恢复后几乎消失。另一方面，在整个实验过程中，丙泊酚诱导的老年大鼠CA1区LTP降低持续存在。此外，TBS诱导的年轻大鼠麻醉后72小时CA1丝状肌动蛋白（F-肌动蛋白）聚合增加和磷酸化丝切蛋白表达增强，2周后这种变化显著减弱。然而，在麻醉的老年大鼠中，CA1区F-肌动蛋白和磷酸化丝切蛋白的改变在实验结束时仍然存在。综上所述，我们的结果表明，年轻和老年大鼠对丙泊酚麻醉的不同反应可能与F-肌动蛋白的不同聚合有关。
