Sun Mang, Yuan Ruixue, Liu Hui, Zhang Jing, Tu Shengfen
Department of Anesthesiology, Children's Hospital of Chongqing Medical University, China.
Chongqing Key Laboratory of Children Urogenital Development and Tissue Engineering, China.
Genes Dis. 2019 Feb 8;7(2):245-252. doi: 10.1016/j.gendis.2019.02.001. eCollection 2020 Jun.
Propofol is widely used as an intravenous drug for induction and maintenance in general anesthesia. Hypoxemia is a common complication during perianesthesia. We want to know the effect of propofol on spatial memory and LTP (Long-term potentiation) under hypoxic conditions. In this study, 84 seven-day-old Sprague-Dawley rats were randomly assigned into six groups (n = 14)-four control groups: lipid emulsion solvent + 50% oxygen (CO), lipid emulsion solvent + room air (CA), lipid emulsion solvent + 18% oxygen (CH), and propofol + 50% oxygen (propofol-oxygen, PO); and two experiment groups: propofol + room air (propofol-air, PA), and propofol + 18% oxygen (propofol-hypoxia, PH). After receiving propofol (50 mg/kg) or the same volume of intralipid intraperitoneal (5.0 ml/kg), injected once per day for seven consecutive days, the rats were exposed to 18% oxygen, 50% oxygen and air, until recovery of the righting reflex. We found that the apoptotic index and activated caspase-3 increased in the PH group (P < 0.05) compared with the PA group, fEPSP (field excitatory postsynaptic) potential and success induction rate of LTP reduced in all propofol groups (P < 0.05). Compared with the PO group, the fEPSP and success induction rate of LTP reduced significantly in the PA and PH groups (P < 0.05). Moreover, compared with CH group, the average time of escape latency was longer, and the number of platform location crossings was significantly reduced in the PH group (P < 0.05). Thus, we believe that adequate oxygen is very important during propofol anesthesia.
丙泊酚作为一种静脉药物，被广泛用于全身麻醉的诱导和维持。低氧血症是围麻醉期的常见并发症。我们想了解在缺氧条件下丙泊酚对空间记忆和长时程增强（LTP）的影响。在本研究中，84只7日龄的Sprague-Dawley大鼠被随机分为6组（n = 14）——4个对照组：脂质乳剂溶剂 + 50%氧气（CO）、脂质乳剂溶剂 + 室内空气（CA）、脂质乳剂溶剂 + 18%氧气（CH）以及丙泊酚 + 50%氧气（丙泊酚-氧气，PO）；2个实验组：丙泊酚 + 室内空气（丙泊酚-空气，PA）以及丙泊酚 + 18%氧气（丙泊酚-缺氧，PH）。连续7天每天腹腔注射丙泊酚（50 mg/kg）或相同体积的脂肪乳（5.0 ml/kg）后，将大鼠暴露于18%氧气、50%氧气和空气中，直至翻正反射恢复。我们发现，与PA组相比，PH组的凋亡指数和活化的半胱天冬酶-3增加（P < 0.05），所有丙泊酚组的场兴奋性突触后电位（fEPSP）和LTP成功诱导率降低（P < 0.05）。与PO组相比，PA组和PH组的fEPSP和LTP成功诱导率显著降低（P < 0.05）。此外，与CH组相比，PH组的逃避潜伏期平均时间更长，平台位置穿越次数显著减少（P < 0.05）。因此，我们认为丙泊酚麻醉期间充足的氧气非常重要。
