Takebayashi Akie, Kimura Fuminori, Kishi Yohei, Ishida Mitsuaki, Takahashi Akimasa, Yamanaka Akiyoshi, Wu Di, Zheng Luyi, Takahashi Kentaro, Suginami Hiroshi, Murakami Takashi
Department of Obstetrics and Gynecology, Shiga University of Medical Science, Otsu, Shiga, Japan.
Am J Reprod Immunol. 2015 Mar;73(3):221-31. doi: 10.1111/aji.12331. Epub 2014 Oct 25.
Endometriosis is recognized as a chronic inflammatory disease and is related to immune response. There have been reports that revealed the different distribution of macrophage within the eutopic endometrium of women with endometriosis. Macrophages are functionally polarized into M1 and M2 cell lineages. We studied a difference in the subpopulations of M1 and M2 macrophages within the eutopic endometrium in patients with or without endometriosis to investigate how the eutopic endometrium is stimulated immunologically.
Thirty-six patients with endometriosis (endometriosis group) and 37 without endometriosis (non-endometriosis group) were analyzed. Paraffin-embedded endometrial specimens were used for the study. Consecutive sections were used for immunostaining of CD68 (pan-macrophage marker) and CD163 (M2 macrophage marker). Cells positive for each marker were quantified, and the ratio of M2 macrophages in pan-macrophages was calculated.
The endometriosis group had a significantly higher number of pan-macrophages than the control group in all phases (P < 0.05). The ratios of M2 macrophages in pan-macrophages were significantly lower in all phases in the endometriosis group (P < 0.05).
The macrophage population slants toward M1 in the endometrium of endometriosis patients. The endometrium appeared to be stimulated by some organelles and/or substances that induce M1 in endometriosis patients.
子宫内膜异位症被认为是一种慢性炎症性疾病，与免疫反应有关。已有报道揭示了子宫内膜异位症患者在位内膜中巨噬细胞的不同分布情况。巨噬细胞在功能上可极化为M1和M2细胞谱系。我们研究了有或无子宫内膜异位症患者在位内膜中M1和M2巨噬细胞亚群的差异，以探讨在位内膜是如何受到免疫刺激的。
分析了36例子宫内膜异位症患者（子宫内膜异位症组）和37例无子宫内膜异位症患者（非子宫内膜异位症组）。采用石蜡包埋的子宫内膜标本进行研究。连续切片用于CD68（全巨噬细胞标志物）和CD163（M2巨噬细胞标志物）的免疫染色。对每种标志物阳性的细胞进行定量，并计算M2巨噬细胞在全巨噬细胞中的比例。
子宫内膜异位症组在各阶段的全巨噬细胞数量均显著高于对照组（P < 0.05）。子宫内膜异位症组各阶段M2巨噬细胞在全巨噬细胞中的比例均显著低于对照组（P < 0.05）。
子宫内膜异位症患者的子宫内膜中巨噬细胞群体倾向于M1型。子宫内膜似乎受到某些细胞器和/或物质的刺激，这些物质在子宫内膜异位症患者中诱导M1型巨噬细胞产生。
