暴露于几种分化剂后，检测弗氏红白血病细胞DNA中5-甲基胞嘧啶的去除情况。

Detection of 5-methylcytosine removal from DNA of Friend erythroleukemia cells following exposure to several differentiating agents.

作者信息

Kuykendall J R, Cox R

机构信息

Cancer Research Laboratory, Veterans Administration Medical Center, Memphis, TN 38104.

出版信息

Cancer Lett. 1989 Sep 15;47(1-2):149-52. doi: 10.1016/0304-3835(89)90191-2.

DOI:10.1016/0304-3835(89)90191-2

PMID:2534571

Abstract

The use of a 32P-labeling procedure has allowed us to monitor the stability of 5-methylcytosine (5-mC) in the existing fraction of DNA in differentiating Friend erythroleukemia cells (FELCs). The levels of 5-mC in existing DNA of control cells remained constant for 20 h, at nearly 3.95% of total labeled cytosine. Exposure of pre-labeled cells to 5 mM N'-methylnicotinamide (N'-MN), 4 mM hexamethylene bisacetamide (HMBA), or 270 mM dimethylsulfoxide (DMSO) over the same time-period resulted in the loss of 6.1, 4.8 and 1.8%, respectively, of the 5-mC from the pool of labeled cytosine present in the existing fraction of the DNA.

摘要

使用³²P标记程序使我们能够监测分化中的弗瑞德红白血病细胞（FELCs）现有DNA片段中5-甲基胞嘧啶（5-mC）的稳定性。对照细胞现有DNA中5-mC的水平在20小时内保持恒定，接近总标记胞嘧啶的3.95%。在相同时间段内，将预先标记的细胞暴露于5 mM N'-甲基烟酰胺（N'-MN）、4 mM六亚甲基双乙酰胺（HMBA）或270 mM二甲基亚砜（DMSO）中，导致DNA现有片段中标记胞嘧啶池中的5-mC分别损失6.1%、4.8%和1.8%。