壳聚糖/PIK3CA小干扰RNA纳米颗粒介导的PIK3CA基因干扰降低胃癌细胞的体外侵袭能力
[Chitosan/PIK3CA siRNA nanoparticle-mediated PIK3CA gene interference decreases the invasive capacity of gastric cancer cells in vitro].
作者信息
Zhou Xinke, He Lu, Liang Min, Liu Jifang
机构信息
Affiliated Tumor Hospital of Guangzhou Medical University, Guangzhou 510095, China.E-mail:
出版信息
Nan Fang Yi Ke Da Xue Xue Bao. 2014 Oct;34(10):1503-6.
OBJECTIVE
To investigate the effect of PIK3CA/siRNA chitosan nanoparticle on the invasiveness of gastric carcinoma and the potential value of PIK3CA/siRNA chitosan nanoparticle in suppressing the metastasis of gastric carcinoma.
METHODS
Gastric cancer cells were treated with PIK3CA/siRNA nanoparticle (with a diameter of 350 nm), and the efficiency of PIK3CA gene interference was evaluated using Western blotting and real-time PCR. The changes of the invasive capacity of the treated cells was assessed with Transwell assay.
RESULTS
PIK3CA/siRNA chitosan nanoparticle efficiently lowered the expression level of PIK3CA and significantly decreased the invasion of BGC823 cells.
CONCLUSION
PIK3CA gene interference mediated by PIK3CA/siRNA chitosan nanoparticle can decrease the invasive capacity of gastric cancer cells in vitro.
目的
探讨PIK3CA/siRNA壳聚糖纳米粒对胃癌侵袭能力的影响以及PIK3CA/siRNA壳聚糖纳米粒在抑制胃癌转移方面的潜在价值。
方法
用PIK3CA/siRNA纳米粒（直径350nm）处理胃癌细胞，采用蛋白质免疫印迹法和实时定量聚合酶链反应评估PIK3CA基因干扰效率。用Transwell实验评估处理后细胞侵袭能力的变化。
结果
PIK3CA/siRNA壳聚糖纳米粒有效降低了PIK3CA的表达水平，并显著降低了BGC823细胞的侵袭能力。
结论
PIK3CA/siRNA壳聚糖纳米粒介导的PIK3CA基因干扰可在体外降低胃癌细胞的侵袭能力。
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