壳聚糖纳米粒介导的 BRAF siRNA 干扰对胃癌细胞侵袭转移的影响。

Effects of chitosan nanoparticle-mediated BRAF siRNA interference on invasion and metastasis of gastric cancer cells.

机构信息

a Xi'an Medical University , Xi'an , P. R. China.

出版信息

Artif Cells Nanomed Biotechnol. 2016 Aug;44(5):1232-5. doi: 10.3109/21691401.2015.1019666. Epub 2015 Mar 20.

DOI:10.3109/21691401.2015.1019666

Abstract

To observe the changes in invasion capacity of gastric cancer BGC823 cells after being treated with chitosan-encapsulated BRAF siRNA nanoparticles, and to evaluate the effects of the nanoparticle-mediated BRAF siRNA interference on cell invasion and metastasis, BRAF siRNA was encapsulated with chitosan into nanoparticles sized 350 nm to treat gastric cancer cells. Silencing of BRAF was detected by Western blot and PCR, and cell invasion was observed by the Transwell assay. The nanoparticles significantly downregulated BRAF expression in BGC823 cells (P < 0.01) and inhibited their invasion (P < 0.001). Chitosan nanoparticle-mediated BRAF siRNA interference evidently reduced the invasion capacity of gastric cancers.

摘要

观察壳聚糖包裹 BRAF siRNA 纳米粒处理胃癌 BGC823 细胞后侵袭能力的变化，评价纳米粒介导 BRAF siRNA 干扰对胃癌细胞侵袭和转移的影响，将 BRAF siRNA 包裹于壳聚糖制成粒径为 350nm 的纳米粒作用于胃癌细胞，Western blot 和 PCR 检测 BRAF 沉默情况，Transwell 小室检测细胞侵袭，纳米粒可显著下调 BGC823 细胞 BRAF 的表达（P<0.01），并抑制其侵袭（P<0.001）。壳聚糖纳米粒介导的 BRAF siRNA 干扰明显降低了胃癌的侵袭能力。