Khairunisa Siti Qamariyah, Kotaki Tomohiro, Witaningrum Adiana Mutamsari, Yunifiar M Muhammad Qushai, Sukartiningrum Septhia Dwi, Kameoka Masanori
1 Indonesian-Japan Collaborative Research Center for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University , Surabaya, Indonesia .
AIDS Res Hum Retroviruses. 2015 Feb;31(2):255-9. doi: 10.1089/AID.2014.0221. Epub 2014 Nov 19.
Although HIV-1 drug resistance is a major obstacle in Indonesia, information on drug resistance is limited. In this study, the viral subtype and appearance of drug resistance mutations in the HIV-1 protease (PR) and reverse transcriptase (RT) genes were determined among drug-treated, HIV-1-infected patients in Surabaya. HIV-1 patients who received antiretroviral therapy (ART) more than 2 years were randomly recruited regardless of the viral load or ART failure. Fifty-eight HIV-1 PR genes and 53 RT genes were sequenced. CRF01_AE viruses were identified as the predominant strain. Major drug resistance mutations were not detected in the PR genes. In contrast, 37.7% (20/53) of the participants had one or more major drug resistance mutations in the RT genes, predominantly M184V (28.3%), K103N (11.3%), and thymidine analogue mutations (TAMs) (20.8%). The high prevalence of drug resistance mutations in RT genes indicated the necessity of monitoring the effectiveness of ART in Indonesia.
尽管HIV-1耐药性是印度尼西亚面临的一个主要障碍，但有关耐药性的信息却很有限。在本研究中，我们确定了泗水接受过药物治疗的HIV-1感染患者中HIV-1蛋白酶（PR）和逆转录酶（RT）基因的病毒亚型及耐药性突变情况。随机招募了接受抗逆转录病毒治疗（ART）超过2年的HIV-1患者，不考虑病毒载量或ART治疗失败情况。对58个HIV-1 PR基因和53个RT基因进行了测序。CRF01_AE病毒被鉴定为主要毒株。在PR基因中未检测到主要耐药性突变。相比之下，37.7%（20/53）的参与者在RT基因中存在一个或多个主要耐药性突变，主要为M184V（28.3%）、K103N（11.3%）和胸苷类似物突变（TAMs）（20.8%）。RT基因中耐药性突变的高流行率表明在印度尼西亚监测ART有效性的必要性。
