McTaggart Lisa R, Doucet Jennifer, Witkowska Maria, Richardson Susan E
Mycology Section, Ontario Public Health Laboratories, Public Health Ontario, Toronto, Ontario, Canada
Mycology Section, Ontario Public Health Laboratories, Public Health Ontario, Toronto, Ontario, Canada.
Antimicrob Agents Chemother. 2015 Jan;59(1):269-75. doi: 10.1128/AAC.02770-14. Epub 2014 Oct 27.
Antimicrobial susceptibility patterns of 112 clinical isolates, 28 type strains, and 9 reference strains of Nocardia were determined using the Sensititre Rapmyco microdilution panel (Thermo Fisher, Inc.). Isolates were identified by highly discriminatory multilocus sequence analysis and were chosen to represent the diversity of species recovered from clinical specimens in Ontario, Canada. Susceptibility to the most commonly used drug, trimethoprim-sulfamethoxazole, was observed in 97% of isolates. Linezolid and amikacin were also highly effective; 100% and 99% of all isolates demonstrated a susceptible phenotype. For the remaining antimicrobials, resistance was species specific with isolates of Nocardia otitidiscaviarum, N. brasiliensis, N. abscessus complex, N. nova complex, N. transvalensis complex, N. farcinica, and N. cyriacigeorgica displaying the traditional characteristic drug pattern types. In addition, the antimicrobial susceptibility profiles of a variety of rarely encountered species isolated from clinical specimens are reported for the first time and were categorized into four additional drug pattern types. Finally, MICs for the control strains N. nova ATCC BAA-2227, N. asteroides ATCC 19247(T), and N. farcinica ATCC 23826 were robustly determined to demonstrate method reproducibility and suitability of the commercial Sensititre Rapmyco panel for antimicrobial susceptibility testing of Nocardia spp. isolated from clinical specimens. The reported values will facilitate quality control and standardization among laboratories.
使用Sensititre Rapmyco微量稀释板（赛默飞世尔科技公司）测定了112株临床分离株、28株模式菌株和9株诺卡菌参考菌株的抗菌药敏模式。通过高分辨率多位点序列分析对分离株进行鉴定，并选择这些分离株以代表从加拿大安大略省临床标本中分离出的菌种多样性。在97%的分离株中观察到对最常用药物复方磺胺甲恶唑的敏感性。利奈唑胺和阿米卡星也非常有效；所有分离株中有100%和99%表现出敏感表型。对于其余抗菌药物，耐药性具有菌种特异性，耳氏诺卡菌、巴西诺卡菌、脓肿诺卡菌复合体、新星诺卡菌复合体、德兰士瓦诺卡菌复合体、嗜皮诺卡菌和乔治亚诺卡菌的分离株呈现出传统的特征性药敏模式类型。此外，首次报告了从临床标本中分离出的多种罕见菌种的抗菌药敏谱，并将其归类为另外四种药敏模式类型。最后，可靠地测定了模式菌株新星诺卡菌ATCC BAA - 2227、星状诺卡菌ATCC 19247(T)和嗜皮诺卡菌ATCC 23826的最低抑菌浓度，以证明该方法的可重复性以及商业Sensititre Rapmyco板对从临床标本中分离的诺卡菌属进行抗菌药敏试验的适用性。报告的值将有助于实验室之间的质量控制和标准化。
