Koshkin F A, Chistyakov D A, Nikitin A G, Konovalov A N, Potapov A A, Usachyov D Yu, Pitskhelauri D I, Kobyakov G L, Shishkina L V, Chekhonin V P
Department of Medical Nanobiotechnologies, N. I. Pirogov Russian Research Medical University, the Ministry of Health of the Russian Federation, Moscow, Russia,
Bull Exp Biol Med. 2014 Oct;157(6):794-7. doi: 10.1007/s10517-014-2669-8. Epub 2014 Oct 29.
The expression profiles of 10 mature microRNA (7, 10a, 17, 20a, 21, 23a, 26a, 137, 222) in biopsy specimens of gliomas of different malignancy were studied by the real time PCR with SYBR Green I fluorescent stain. The expression of microRNA-21 increased significantly, while that of microRNA-137 decreased, depending on the tumor malignancy. The expression of microRNA-9, -17, -20a, -23a, -26a was significantly higher, while that of microRNA-7 lower in the tumor vs. control tissue samples. New data on the molecular pathological mechanisms of gliomas related to their malignancy were obtained. Quantitative analysis of microRNA was suggested as a potential diagnostic marker of brain tumors.
采用SYBR Green I荧光染色实时定量PCR技术，研究了10种成熟微小RNA（7、10a、17、20a、21、23a、26a、137、222）在不同恶性程度胶质瘤活检标本中的表达谱。微小RNA-21的表达显著增加，而微小RNA-137的表达则随肿瘤恶性程度降低。与对照组织样本相比，肿瘤中微小RNA-9、-17、-20a、-23a、-26a的表达显著升高，而微小RNA-7的表达较低。获得了与胶质瘤恶性程度相关的分子病理机制的新数据。微小RNA的定量分析被认为是脑肿瘤潜在的诊断标志物。
