Department of Microbiology and Immunology Programme, National University of Singapore (NUS), Life Sciences Institute, #03-05, 28 Medical Drive, 117456 (Singapore).
Angew Chem Int Ed Engl. 2014 Dec 1;53(49):13390-4. doi: 10.1002/anie.201406295. Epub 2014 Oct 27.
Bioorthogonal cleavable linkers are attractive building blocks for compounds that can be manipulated to study biological and cellular processes. Sodium dithionite sensitive azobenzene-containing (Abc) peptides were applied for the temporary stabilization of recombinant MHC complexes, which can then be employed to generate libraries of MHC tetramers after exchange with a novel epitope. This technology represents an important tool for high-throughput studies of disease-specific T cell responses.
生物正交可裂解连接子是一类很有吸引力的构建模块，它们可以被修饰以研究生物和细胞过程。含二硫苏糖醇敏感偶氮苯的肽（Abc 肽）被应用于暂时稳定重组 MHC 复合物，然后可以通过与新表位交换来生成 MHC 四聚体文库。这项技术是高通量研究疾病特异性 T 细胞反应的重要工具。
