Kaiser C J, Ksander B R, Streilein J W
Department of Immunology and Microbiology, University of Miami School of Medicine, FL 33101.
Reg Immunol. 1989 Jan-Feb;2(1):42-9.
Antigens introduced into the anterior chamber (AC) of the eye elicit systemic, antigen-specific suppression of delayed hypersensitivity combined with primed cytotoxic T cells and elevated levels of serum antibodies, a unique immune response termed anterior chamber associated immune deviation (ACAID). Among the mechanisms that have been implicated in the induction of this response is the possibility that T cells first recognize antigen within the AC, where-upon they then escape to initiate the induction of systemic suppression. Because this possibility implies that T-cell recognition of antigen could occur in the aqueous humor (AqH) that normally fills the AC, we tested the effects of freshly obtained AqH on lymphocyte proliferative responses in vitro. The results indicate that AqH from mice and rabbits profoundly inhibits (1) T-lymphocyte proliferation to antigens, (2) T- and B-lymphocyte responses to polyclonal mitogens, and (3) growth-factor-driven lymphocyte proliferation. The antiproliferative activity of AqH was also effective on some, but not all, neoplastic cells. The activity was shown to be neither species specific nor directly cytotoxic to cells. The activity was distinct from the growth inhibitory effects of normal mouse serum. We conclude that AqH contains a soluble factor(s) that is a potent inhibitor of cell proliferation. The potential role(s) of this activity in ocular wound healing and in intraocular immune responses are discussed.
引入眼房水（AC）的抗原可引发全身性、抗原特异性的迟发型超敏反应抑制，同时伴有致敏细胞毒性T细胞和血清抗体水平升高，这是一种独特的免疫反应，称为房水相关免疫偏离（ACAID）。在引发这种反应的机制中，有一种可能性是T细胞首先在AC内识别抗原，随后它们逃逸以启动全身性抑制的诱导。由于这种可能性意味着T细胞对抗原的识别可能发生在通常充满AC的房水（AqH）中，我们测试了新鲜获取的AqH对体外淋巴细胞增殖反应的影响。结果表明，来自小鼠和兔子的AqH可显著抑制：（1）T淋巴细胞对抗原的增殖；（2）T淋巴细胞和B淋巴细胞对多克隆有丝分裂原的反应；（3）生长因子驱动的淋巴细胞增殖。AqH的抗增殖活性对一些但并非所有肿瘤细胞也有效。该活性既非物种特异性，对细胞也无直接细胞毒性。该活性不同于正常小鼠血清的生长抑制作用。我们得出结论，AqH含有一种可溶性因子，它是细胞增殖的有效抑制剂。本文讨论了这种活性在眼伤口愈合和眼内免疫反应中的潜在作用。
