Keio University School of Medicine, Tokyo 160-8582, Japan.
Division of Pulmonary Medicine, Department of Medicine, Keio University School of Medicine, Tokyo 160-8582, Japan.
Int J Oncol. 2015 Jan;46(1):423-9. doi: 10.3892/ijo.2014.2718. Epub 2014 Oct 21.
Cancer cells are surrounded by the extracellular fluid, matrix, and stroma cells. Little is known about how extracellular components such as growth factor ligands affect the biology of lung cancer cells. The objective of this study was to determine whether extracellular fibroblast growth factors (FGFs) can affect the biology of lung cancer cells and to understand how extracellular FGFs affect the biology of lung cancer cells, including non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC) cells. Out of the 23 reported FGFs, we focused on FGF2, FGF9 and FGF10. We examined the effect of FGFs on proliferation, treatment sensitivity, and apoptosis of NSCLC (PC9) and SCLC (H69, H82 and H146) cells in vitro. To determine which FGF was the most clinically relevant, we also examined FGF2 and FGF9 concentrations in the serum of patients with lung cancer. We found that extracellular FGFs can affect proliferation, treatment sensitivity, and apoptosis of lung cancer cells in a cell-specific manner. Our results indicate that extracellular FGFs affect the biology of lung cancer cells through multiple functions.
癌细胞被细胞外液、基质和基质细胞所包围。目前人们对于生长因子配体等细胞外成分如何影响肺癌细胞的生物学特性知之甚少。本研究旨在探讨细胞外成纤维细胞生长因子（FGFs）是否能影响肺癌细胞的生物学特性，以及了解细胞外 FGFs 如何影响肺癌细胞的生物学特性，包括非小细胞肺癌（NSCLC）和小细胞肺癌（SCLC）细胞。在已报道的 23 种 FGF 中，我们重点研究了 FGF2、FGF9 和 FGF10。我们检测了 FGFs 对 NSCLC（PC9）和 SCLC（H69、H82 和 H146）细胞体外增殖、治疗敏感性和凋亡的影响。为了确定哪种 FGF 最具临床相关性，我们还检测了肺癌患者血清中 FGF2 和 FGF9 的浓度。结果表明，细胞外 FGFs 以细胞特异性方式影响肺癌细胞的增殖、治疗敏感性和凋亡。我们的研究结果表明，细胞外 FGFs 通过多种功能影响肺癌细胞的生物学特性。
