小麦核糖体RNA转录本的定位
Mapping of ribosomal RNA transcripts in wheat.
作者信息
Vincentz M, Flavell R B
机构信息
AFRC Institute of Plant Science Research, Cambridge Laboratory, Trumpington, United Kingdom.
出版信息
Plant Cell. 1989 Jun;1(6):579-89. doi: 10.1105/tpc.1.6.579.
Abstract
Ribosomal RNA transcripts in wheat have been studied by RNA gel blotting and their termini determined from electrophoretic analysis of S1 nuclease-resistant RNA/DNA hybrids and also of hybrids created by primer extension. A major putative transcription initiation site has been localized 1132 base pairs upstream from the 5' end of the 18S RNA sequence. A major putative processing site occurs 640 base pairs from the 5' end of the 18S RNA. Transcripts extending at least 750 base pairs beyond the 3' end of the 25S rRNA sequence into the array of intergenic repeats are present, as are transcripts covering the principal initiation sequence. This suggests that some transcripts extend through the intergenic DNA from one repeat unit into the next.
摘要
通过RNA凝胶印迹法研究了小麦中的核糖体RNA转录本，并通过对S1核酸酶抗性RNA/DNA杂交体以及引物延伸产生的杂交体进行电泳分析来确定其末端。一个主要的假定转录起始位点位于18S RNA序列5'端上游1132个碱基对处。一个主要的假定加工位点位于18S RNA 5'端640个碱基对处。存在延伸到25S rRNA序列3'端至少750个碱基对进入基因间重复序列阵列的转录本，以及覆盖主要起始序列的转录本。这表明一些转录本从一个重复单元穿过基因间DNA延伸到下一个重复单元。
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