谷胱甘肽S-转移酶P1（GSTP1）使膀胱癌细胞阻滞于G0/G1期并上调p21表达。
GSTP1 arrests bladder cancer T24 cells in G0/G1 phase and up-regulates p21 expression.
作者信息
Gao Li, Fang You-Qiang, Zhang Tian-Yu, Ge Bo, Xu Bin, Huang Jie-Fu, Zhang Zhen-Feng, Tan Ning
机构信息
Department of Urology, Affiliated Hospital, Guilin Medical College Guilin 541004, Guangxi, China.
Department of Urology, The Third Affiliated Hospital, Sun Yat-Sen University Guangzhou 510630, China.
出版信息
Int J Clin Exp Med. 2014 Sep 15;7(9):2984-91. eCollection 2014.
OBJECTIVE
GSTP1 over-expression was introduced into human bladder cancer T24 cells via the lentivirus system. The influence of GSTP1 on the proliferation and cell cycle of T24 cells as well as the potential mechanisms was investigated.
METHODS
The lentiviral vector GSTP1-pWPXL was constructed and transfected into T24 cells in the presence of Lipofectamine 2000. CCK8 assay and colony formation test were performed to explore the impact of GSTP1 on the proliferation of T24 cells. Ollowing PI staining, flow cytometry was done to detect the proportion of T24 cells in different phases. Western blot assay was conducted to detect the protein p21 expression.
RESULTS
When compared with control group, T24 cells with GSTP1 over-expression showed significant reduction in cell proliferation (P < 0.01) and they were arrested in G0/G1 phase. Western blot assay indicated that the p21 protein expression in T24 cells with GSTP1 over-expression was significantly higher than that in control group.
CONCLUSION
GSTP1 may inhibit the proliferation of T24 cells and arrest these cells in G0/G1 phase, which may be ascribed to the up-regulated expression of p21.
目的
通过慢病毒系统将GSTP1过表达导入人膀胱癌T24细胞。研究GSTP1对T24细胞增殖和细胞周期的影响及其潜在机制。
方法
构建慢病毒载体GSTP1-pWPXL，并在Lipofectamine 2000存在的情况下转染至T24细胞。进行CCK8测定和集落形成试验，以探讨GSTP1对T24细胞增殖的影响。进行PI染色后，采用流式细胞术检测不同时期T24细胞的比例。进行蛋白质印迹分析以检测p21蛋白的表达。
结果
与对照组相比，GSTP1过表达的T24细胞增殖显著降低（P < 0.01），且细胞停滞于G0/G1期。蛋白质印迹分析表明，GSTP1过表达的T24细胞中p21蛋白表达显著高于对照组。
结论
GSTP1可能抑制T24细胞的增殖并使这些细胞停滞于G0/G1期，这可能归因于p21表达上调。
Zotero 插件