Dahlgren C, Johansson A, Orselius K
Department of Medical Microbiology, University of Linköping, Sweden.
Biochim Biophys Acta. 1989 Jan 17;1010(1):41-8. doi: 10.1016/0167-4889(89)90182-1.
The role of subcellular granule in the capacity to generate reactive oxygen metabolites in human granulocytes was studied using normal cells and organell-free neutrophil cytoplasts. The cytoplasts are devoid of granules but have an intact ligand-receptor coupling mechanism. Both the chemotactic peptide formylmethionylleucylphenylalanine (FMLP) and the ionophore ionomycin induced a chemiluminescence response in normal cells, but only FMLP stimulation was associated with any notable hydrogen peroxide production. However, in the presence of azide, a potent inhibitor of the hydrogen peroxide-consuming enzymes, catalase and myeloperoxidase, a pronounced release of hydrogen peroxide was also induced by ionomycin. The response of cytoplasts to FMLP proceeded with a rate and time-course similar to those seen in intact cells, whereas in response to ionomycin they produced very low quantities of hydrogen peroxide, even in the presence of azide. Analysis of the data presented in this study leads to the following conclusion: (i) FMLP, which acts through cell surface receptors, causes the cells to produce oxygen metabolites, which, to a large extent, are released from the cells, a process that is not dependent on subcellular granule; and (ii) ionomycin, which bypasses cell surface receptors, is also capable of stimulating hydrogen peroxide formation that is granule-dependent and that is retained inside the cells.
利用正常细胞和无细胞器的中性粒细胞胞质体，研究了亚细胞颗粒在人类粒细胞中产生活性氧代谢产物能力中的作用。胞质体没有颗粒，但具有完整的配体-受体偶联机制。趋化肽甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）和离子载体离子霉素均可诱导正常细胞产生化学发光反应，但只有FMLP刺激与显著的过氧化氢产生有关。然而，在叠氮化物（一种过氧化氢消耗酶过氧化氢酶和髓过氧化物酶的有效抑制剂）存在的情况下，离子霉素也能诱导过氧化氢的大量释放。胞质体对FMLP的反应速率和时间进程与完整细胞中的相似，而对离子霉素的反应，即使在叠氮化物存在的情况下，它们产生的过氧化氢量也非常低。对本研究中呈现的数据进行分析可得出以下结论：（i）通过细胞表面受体起作用的FMLP会导致细胞产生氧代谢产物，这些产物在很大程度上从细胞中释放出来，这一过程不依赖于亚细胞颗粒；（ii）绕过细胞表面受体的离子霉素也能够刺激依赖颗粒且保留在细胞内的过氧化氢形成。
