Dahlgren C
Department of Medical Microbiology, Linköping University, School of Medicine, Sweden.
J Biolumin Chemilumin. 1988 Jan-Mar;2(1):25-33. doi: 10.1002/bio.1170020106.
When neutrophil cytoplasts (granule-free vesicles of cytoplasm enclosed by plasmalemma) were exposed to the chemotactic peptide formylmethionyl-leucyl-phenylalanine, no luminol-dependent chemiluminescence was detected, despite a pronounced production of superoxide anions and hydrogen peroxide. Addition of purified myeloperoxidase (MPO) or human serum albumin (HSA) to the cytoplasts before the stimulus resulted in a chemiluminescence response. In contrast to the bimodal response obtained from normal PMNL, only a single peak of chemiluminescence was obtained from the cytoplasts responding to the peptide. The time-course of the response obtained in the presence of albumin was more prolonged than the response obtained in the presence of MPO. Furthermore, the involvement of different oxidative metabolites in the MPO and the HSA systems, respectively, was demonstrated by the accumulated chemiluminescence effect obtained when MPO, but not HSA, was introduced in the measuring system after FMLP addition. From these results it can be concluded that there are at least two different light-generating mechanisms in FMLP-induced luminol-dependent chemiluminescence of neutrophil cytoplasts. One of these is dependent on myeloperoxidase and possibly related to the myeloperoxidase-hydrogen peroxide reaction, whereas the other one is hydrogen peroxide independent.
当中性粒细胞胞质体（由质膜包裹的无颗粒细胞质囊泡）暴露于趋化肽甲酰甲硫氨酰-亮氨酰-苯丙氨酸时，尽管超氧阴离子和过氧化氢大量产生，但未检测到鲁米诺依赖性化学发光。在刺激前向胞质体中添加纯化的髓过氧化物酶（MPO）或人血清白蛋白（HSA）会导致化学发光反应。与从正常多形核白细胞获得的双峰反应不同，对该肽作出反应的胞质体仅获得一个化学发光峰。在白蛋白存在下获得的反应的时间进程比在MPO存在下获得的反应更持久。此外，当在添加FMLP后将MPO而非HSA引入测量系统时获得的累积化学发光效应表明，MPO和HSA系统分别涉及不同的氧化代谢产物。从这些结果可以得出结论，在FMLP诱导的中性粒细胞胞质体的鲁米诺依赖性化学发光中至少存在两种不同的发光机制。其中一种依赖于髓过氧化物酶，可能与髓过氧化物酶-过氧化氢反应有关，而另一种则与过氧化氢无关。
