Efficient sample processing for proteomics applications--are we there yet?

作者信息

Kanshin Evgeny, Thibault Pierre

机构信息

Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada.

Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Chemistry, Université de Montréal, Montréal, QC, Canada.

出版信息

Mol Syst Biol. 2014 Oct 30;10(10):758. doi: 10.15252/msb.20145760.

DOI:10.15252/msb.20145760

PMID:25358342

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4299379/

Abstract

The ability to solubilize and digest protein extracts and recover peptides with high efficiency is of paramount importance in proteomics. A novel proteomic sample preparation protocol by Krijgsveld and colleagues (Hughes 2014) provides significant advantages by enabling all sample processing steps to be carried out in a single tube to minimize sample losses, thereby enhancing sensitivity, throughput, and scalability of proteomics analyses.

摘要