Kanshin Evgeny, Thibault Pierre
Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada.
Institute for Research in Immunology and Cancer, Université de Montréal, Montréal, QC, Canada Department of Chemistry, Université de Montréal, Montréal, QC, Canada.
Mol Syst Biol. 2014 Oct 30;10(10):758. doi: 10.15252/msb.20145760.
The ability to solubilize and digest protein extracts and recover peptides with high efficiency is of paramount importance in proteomics. A novel proteomic sample preparation protocol by Krijgsveld and colleagues (Hughes 2014) provides significant advantages by enabling all sample processing steps to be carried out in a single tube to minimize sample losses, thereby enhancing sensitivity, throughput, and scalability of proteomics analyses.
在蛋白质组学中，高效溶解和消化蛋白质提取物并回收肽段的能力至关重要。Krijgsveld及其同事提出的一种新型蛋白质组学样品制备方案（Hughes，2014年）具有显著优势，它能使所有样品处理步骤在单个试管中进行，以尽量减少样品损失，从而提高蛋白质组学分析的灵敏度、通量和可扩展性。
