Department of Biology and Biochemistry, University of Bath, Claverton Down, Bath, UK.
J Proteomics. 2010 Oct 10;73(11):2171-85. doi: 10.1016/j.jprot.2010.09.002. Epub 2010 Sep 15.
Advances in mass spectrometry technology, high-throughput proteomics and genome annotations have resulted in significant increases in our molecular understanding of sperm composition. Using improved separation and detection methods and an updated genome annotation, a re-analysis of the Drosophila melanogaster sperm proteome (DmSP) has resulted in the identification of 956 sperm proteins. Comparative analysis with our previous proteomic dataset revealed 766 new proteins and an updated sperm proteome containing a total of 1108 proteins, termed the DmSP-II. This expanded dataset includes additional proteins with predicted sperm functions and confirms previous findings concerning the genomic organization of sperm loci. Bioinformatic and protein network analyses demonstrated high quality and reproducibility of proteome coverage across three replicate mass spectrometry runs. The use of whole-cell proteomics in conjunction with characterized phenotypes, functional annotations and pathway information has advanced our systems level understanding of sperm proteome functional networks.
质谱技术、高通量蛋白质组学和基因组注释的进步，使我们对精子组成的分子认识有了显著提高。利用改良的分离和检测方法以及更新的基因组注释，对黑腹果蝇精子蛋白质组（DmSP）进行重新分析，鉴定出 956 种精子蛋白。与我们之前的蛋白质组数据集进行比较分析，发现了 766 种新蛋白质，以及一个包含总共 1108 种蛋白质的更新的精子蛋白质组，称为 DmSP-II。这个扩展数据集包括了具有预测精子功能的额外蛋白质，并证实了先前关于精子基因座基因组组织的发现。生物信息学和蛋白质网络分析表明，三个重复的质谱运行的蛋白质组覆盖具有高质量和可重复性。全细胞蛋白质组学与已鉴定的表型、功能注释和途径信息的结合使用，提高了我们对精子蛋白质组功能网络的系统水平认识。
