Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha Kashiwa, Chiba, 277-8562, Japan.
J Integr Plant Biol. 2015 Jan;57(1):127-35. doi: 10.1111/jipb.12304.
Vacuoles are suggested to play crucial roles in plant defense-related cell death. During programmed cell death, previous live cell imaging studies have observed vacuoles to become simpler in structure and have implicated this simplification as a prelude to the vacuole's rupture and consequent lysis of the plasma membrane. Here, we examined dynamics of the vacuole in cell cycle-synchronized tobacco BY-2 (Nicotiana tabacum L. cv. Bright Yellow 2) cells during cell death induced by application of culture filtrates of Erwinia carotovora. The filtrate induced death in about 90% of the cells by 24 h. Prior to cell death, vacuole shape simplified and endoplasmic actin filaments disassembled; however, the vacuoles did not rupture until after plasma membrane integrity was lost. Instead of facilitating rupture, the simplification of vacuole structure might play a role in the retrieval of membrane components needed for defense-related cell death.
液泡被认为在植物防御相关的细胞死亡中发挥着关键作用。在程序性细胞死亡过程中，先前的活细胞成像研究观察到液泡的结构变得更加简单，并暗示这种简化是液泡破裂和随后质膜裂解的前奏。在这里，我们检查了在烟草 BY-2（Nicotiana tabacum L. cv. Bright Yellow 2）细胞周期同步化细胞死亡过程中液泡的动态，该细胞死亡是由施用胡萝卜软腐欧文氏菌培养滤液引起的。滤液在 24 小时内导致约 90%的细胞死亡。在细胞死亡之前，液泡形状简化，内质网肌动蛋白丝解体；然而，直到质膜完整性丧失后，液泡才破裂。液泡结构的简化并没有促进破裂，反而可能在回收与防御相关的细胞死亡所需的膜成分方面发挥作用。
