Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, P.R. China.
ACS Appl Mater Interfaces. 2014 Dec 10;6(23):21346-54. doi: 10.1021/am5063025. Epub 2014 Nov 12.
Fabrication of various efficient enzyme reactors has triggered increasing interests for its extensive applications in biological and clinical research. In this study, magnetic nanoparticles were functionalized by a biocompatible reactive polymer, poly(2-vinyl-4,4-dimethylazlactone), which was synthesized by reversible addition-fragmentation chain transfer polymerization. Then, the prepared polymer-modified magnetic nanoparticles were employed as favorable carriers for enzyme immobilization. l-Asparaginase was selected as the model enzyme to fabricate the enzyme reactor, and the prepared enzyme reactor exhibited high loading capacity of 318.0 μg mg(-1) magnetic nanoparticle. Interestingly, it has been observed that the enzymolysis efficiency increased slightly with the lengthened polymer chain, resulting from the increased immobilization amount of enzyme. Meanwhile, the immobilized enzyme could retain more than 95.7% activity after 10 repeated uses and maintain more than 72.6% activity after 10 weeks storage. Moreover, an extracorporeal shunt system was simulated to estimate the potential application capability of the prepared l-asparaginase reactor in acute lymphoblastic leukemia treatment.
制备各种高效的酶反应器引起了人们越来越多的兴趣，因为它在生物和临床研究中有广泛的应用。在这项研究中，磁性纳米粒子通过一种生物相容性的反应性聚合物聚（2-乙烯基-4,4-二甲基恶唑啉）进行功能化，该聚合物通过可逆加成-断裂链转移聚合合成。然后，将制备的聚合物修饰的磁性纳米粒子用作酶固定化的理想载体。选择天冬酰胺酶作为模型酶来制备酶反应器，所制备的酶反应器具有 318.0 μg mg(-1)磁性纳米粒子的高负载能力。有趣的是，观察到随着聚合物链的延长，酶解效率略有提高，这是由于酶的固定化量增加所致。同时，固定化酶在 10 次重复使用后仍保留超过 95.7%的活性，在 10 周储存后仍保留超过 72.6%的活性。此外，模拟了体外分流系统以评估制备的天冬酰胺酶反应器在急性淋巴细胞白血病治疗中的潜在应用能力。
