Rafeeq Hamza, Hussain Asim, Tarar Muhammad Haseeb Anwar, Afsheen Nadia, Bilal Muhammad, Iqbal Hafiz M N
Department of Biochemistry, Riphah International University, Faisalabad, Pakistan.
Department of Biochemistry, University of Agriculture Faisalabad, Faisalabad, Pakistan.
3 Biotech. 2021 Oct;11(10):453. doi: 10.1007/s13205-021-02999-y. Epub 2021 Oct 1.
l-asparaginase is an essential enzyme in medicine and a well-known chemotherapeutic agent. This enzyme's importance is not limited to its use as an anti-cancer agent; it also has a wide variety of medicinal applications. Antimicrobial properties, prevention of infectious disorders, autoimmune diseases, and canine and feline cancer are among the applications. Apart from the healthcare industry, its importance has been identified in the food industry as a food manufacturing agent to lower acrylamide levels. When isolated from their natural habitats, they are especially susceptible to different denaturing conditions due to their protein composition. The use of an immobilization technique is one of the most common approaches suggested to address these limitations. Immobilization is a technique that involves fixing enzymes to or inside stable supports, resulting in a heterogeneous immobilized enzyme framework. Strong support structures usually stabilize the enzymes' configuration, and their functions are maintained as a result. In recent years, there has been a lot of curiosity and focus on the ability of immobilized enzymes. The nanomaterials with ideal properties can be used to immobilize enzymes to regulate key factors that determine the efficacy of bio-catalysis. With applications in biotechnology, immunosensing, biomedicine, and nanotechnology sectors have opened a realm of opportunities for enzyme immobilization.
L-天冬酰胺酶是医学中的一种重要酶，也是一种著名的化疗药物。这种酶的重要性不仅限于其作为抗癌剂的用途；它还具有广泛的医学应用。抗菌特性、预防感染性疾病、自身免疫性疾病以及犬猫癌症等都在其应用范围内。除了医疗保健行业，其在食品工业中作为降低丙烯酰胺水平的食品制造剂的重要性也已得到确认。当从其自然栖息地分离出来时，由于其蛋白质组成，它们特别容易受到不同变性条件的影响。使用固定化技术是解决这些局限性最常用的方法之一。固定化是一种将酶固定在稳定载体上或载体内部的技术，从而形成异质固定化酶框架。坚固的支撑结构通常能稳定酶的构型，其功能也因此得以维持。近年来，人们对固定化酶的能力充满了好奇并给予了关注。具有理想特性的纳米材料可用于固定酶，以调节决定生物催化效果的关键因素。随着在生物技术中的应用，免疫传感、生物医学和纳米技术领域为酶固定化开辟了一系列机会。
