PALMsiever：一种将原始数据转化为单分子定位显微镜结果的工具。
PALMsiever: a tool to turn raw data into results for single-molecule localization microscopy.
作者信息
Pengo Thomas, Holden Seamus J, Manley Suliana
机构信息
Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.
Design of Biological Systems, Systems Biology, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, Advanced Light Microscopy Unit, Center for Genomic Regulation, Dr. Aiguader 88, 08003 Barcelona, Spain, and Laboratory for Experimental Biophysics, Institute for Physics of Biological Systems, School of Basic Sciences, Swiss Federal School of Technology, 1015 Lausanne, Switzerland.
出版信息
Bioinformatics. 2015 Mar 1;31(5):797-8. doi: 10.1093/bioinformatics/btu720. Epub 2014 Oct 31.
During the past decade, localization microscopy (LM) has transformed into an accessible, commercially available technique for life sciences. However, data processing can be challenging to the non-specialist and care is still needed to produce meaningful results. PALMsiever has been developed to provide a user-friendly means of visualizing, filtering and analyzing LM data. It includes drift correction, clustering, intelligent line profiles, many rendering algorithms and 3D data visualization. It incorporates the main analysis and data processing modalities used by experts in the field, as well as several new features we developed, and makes them broadly accessible. It can easily be extended via plugins and is provided as free of charge open-source software.
在过去十年中，定位显微镜技术（LM）已转变为一种生命科学领域中人人可用的商业化技术。然而，数据处理对于非专业人员来说可能具有挑战性，并且仍需要谨慎操作才能得出有意义的结果。PALMsiever的开发旨在提供一种用户友好的方式来可视化、过滤和分析定位显微镜数据。它包括漂移校正、聚类、智能线轮廓、多种渲染算法以及三维数据可视化。它整合了该领域专家使用的主要分析和数据处理方式，以及我们开发的一些新功能，并使其广泛可用。它可以通过插件轻松扩展，并且作为免费的开源软件提供。
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