Raines D E, Cafiso D S
Department of Chemistry, University of Virginia, Charlottesville 22901.
Anesthesiology. 1989 Jan;70(1):57-63. doi: 10.1097/00000542-198901000-00013.
When pH gradients are created across model lipid membranes, there is a well-documented electrogenic flow of protons, which results in the development of a transmembrane potential. As a result, protons come to electrochemical equilibrium across lipid vesicles within tens of minutes. The ability of a series of inhalation anesthetics to enhance the conduction of protons across model membranes was examined. When clinically relevant concentrations of halothane, isoflurane, enflurane, or chloroform were equilibrated with sonicated lipid vesicles, significant increases in the proton conduction were found. However, for even high anesthetic concentrations (above 8 MAC) of ether and cyclopropane, no increases in proton conduction could be detected. These results rule out a common mechanism for general anesthesia involving enhanced proton conduction across membranes.
当在模型脂质膜上形成pH梯度时，质子会发生有充分记录的生电流动，这会导致跨膜电位的产生。结果，质子会在数十分钟内在脂质囊泡内达到电化学平衡。研究了一系列吸入麻醉剂增强质子跨模型膜传导的能力。当将临床相关浓度的氟烷、异氟烷、恩氟烷或氯仿与经超声处理的脂质囊泡平衡时，发现质子传导显著增加。然而，即使对于高浓度麻醉剂（高于8倍最低肺泡有效浓度）的乙醚和环丙烷，也未检测到质子传导增加。这些结果排除了全身麻醉涉及增强质子跨膜传导的常见机制。
