Kim J H, Rimmer J, Mrad N, Ahmadzada S, Harvey R J
Rhinology and Skull Base Research Group,St. Vincent's Centre for Applied Medical Research,Darlinghurst,New South Wales,Australia.
St. Vincent's Hospital Sydney,Darlinghurst,New South Wales,Australia.
J Laryngol Otol. 2015 Jan;129 Suppl 1:S45-50. doi: 10.1017/S0022215114002746. Epub 2014 Nov 3.
This study investigated the effect of Betadine on ciliated human respiratory epithelial cells.
Epithelial cells from human sinonasal mucosa were cultured at the air-liquid interface. The cultures were tested with Hanks' balanced salt solution containing 10 mM HEPES (control), 100 µM ATP (positive control), 5 per cent Betadine or 10 per cent Betadine (clinical dose). Ciliary beat frequency was analysed using a high-speed camera on a computer imaging system.
Undiluted 10 per cent Betadine (n = 6) decreased the proportion of actively beating cilia over 1 minute (p < 0.01). Ciliary beat frequency decreased from 11.15 ± 4.64 Hz to no detectable activity. The result was similar with 5 per cent Betadine (n = 7), with no significant difference compared with the 10 per cent solution findings.
Betadine, at either 5 and 10 per cent, was ciliotoxic. Caution should be applied to the use of topical Betadine solution on the respiratory mucosal surface.
本研究调查了碘伏对人呼吸道纤毛上皮细胞的影响。
将人鼻窦黏膜的上皮细胞在气液界面进行培养。培养物分别用含有10 mM HEPES的汉克斯平衡盐溶液（对照）、100 µM ATP（阳性对照）、5%碘伏或10%碘伏（临床剂量）进行测试。使用计算机成像系统上的高速相机分析纤毛摆动频率。
未稀释的10%碘伏（n = 6）在1分钟内降低了活跃摆动纤毛的比例（p < 0.01）。纤毛摆动频率从11.15 ± 4.64 Hz降至无法检测到的活动。5%碘伏（n = 7）的结果与之相似，与10%溶液的结果相比无显著差异。
5%和10%的碘伏均具有纤毛毒性。在呼吸道黏膜表面使用局部碘伏溶液时应谨慎。
