Department of Periodontology, Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
Department of Nanomedicine (DNP), Graduate School of Medical and Dental Science, Tokyo Medical and Dental University Tokyo, Japan.
Front Cell Dev Biol. 2014 May 21;2:19. doi: 10.3389/fcell.2014.00019. eCollection 2014.
Bone marrow-derived cells (BMCs) are considered to be a major source of mesenchymal stem cells (MSCs) in adults and are known to be effective in periodontal tissue regeneration. However, whether endogenous BMCs are involved in periodontal tissue repair process is uncertain. We therefore created periodontal tissue defects in the buccal alveolar bone of mandibular first molars in bone marrow chimeric mice, and immunohistochemically examined the expression of stromal cell derived factor-1 (SDF-1) and the mobilization of BMCs. We found that SDF-1 expression was increased around the defects at as early as 1 week after injury and that BMCs were mobilized to the defects, while GFP+/CD45+ were rarely observed. Fluorescence-activated cell sorting (FACS) analysis demonstrated that the number of platelet-derived growth factor receptor (pdgfr) α+/Sca-1+ (PαS) cells in the bone marrow decreased after injury. Taken together, these results suggest that BMCs are mobilized to the periodontal tissue defects. Recruitment of BMCs, including a subset of MSCs could be a new target of periodontal treatment.
骨髓来源的细胞（BMCs）被认为是成人间充质干细胞（MSCs）的主要来源，并且已知在牙周组织再生中有效。然而，内源性 BMCs 是否参与牙周组织修复过程尚不确定。因此，我们在骨髓嵌合小鼠的下颌第一磨牙颊侧牙槽骨中创建了牙周组织缺损，并通过免疫组织化学检查基质细胞衍生因子-1（SDF-1）的表达和 BMC 的动员。我们发现，SDF-1 的表达在损伤后 1 周内就增加了，并且 BMC 被动员到了缺陷处，而 GFP+/CD45+则很少观察到。荧光激活细胞分选（FACS）分析表明，损伤后骨髓中血小板衍生生长因子受体（pdgfr）α+/Sca-1+（PαS）细胞的数量减少。综上所述，这些结果表明 BMC 被动员到牙周组织缺损处。BMC 的募集，包括 MSCs 的一个亚群，可能是牙周治疗的一个新靶点。
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