Orofacial Development and Regeneration, Institute of Oral Biology, Medical Faculty, University of Zurich, 8032 Zurich, Switzerland.
Department of Medical Oncology and Hematology, University Hospital of Zurich, 8091 Zurich, Switzerland.
Int J Mol Sci. 2021 Mar 16;22(6):3024. doi: 10.3390/ijms22063024.
Specific stem cell populations within dental mesenchymal tissues guarantee tooth homeostasis and regeneration throughout life. The decision between renewal and differentiation of stem cells is greatly influenced by interactions with stromal cells and extracellular matrix molecules that form the tissue specific stem cell niches. The chemokine is a general marker of stromal cells and plays fundamental roles in the maintenance, mobilization and migration of stem cells. The aim of this study was to exploit -GFP transgenic mice to study the expression patterns of in putative dental niches of intact and injured teeth. We showed that endothelial and stromal cells expressed in the dental pulp tissue of both intact molars and incisors. Isolated non-endothelial dental pulp cells cultured in different conditions in vitro exhibited expression of both adipogenic and osteogenic markers, thus suggesting that these cells possess multipotent fates. Taken together, our results show that is widely expressed in intact and injured teeth and highlight its importance as a key component of the various dental mesenchymal stem cell niches.
特定的干细胞群体存在于牙间质组织中，保证了牙齿在整个生命周期中的稳态和再生。干细胞的更新和分化的决定受到与基质细胞和细胞外基质分子相互作用的极大影响，这些分子形成了组织特异性的干细胞龛。趋化因子是基质细胞的一般标志物，在干细胞的维持、动员和迁移中发挥着基本作用。本研究旨在利用 -GFP 转基因小鼠研究在完整和受损牙齿的潜在牙龛中 的表达模式。我们发现，内皮细胞和基质细胞在完整磨牙和切牙的牙髓组织中均表达 。在体外不同条件下培养分离的非内皮牙髓细胞表现出脂肪生成和成骨标记物的表达，这表明这些细胞具有多能性。总之，我们的结果表明， 在完整和受损的牙齿中广泛表达，并强调其作为各种牙间质干细胞龛的关键组成部分的重要性。
