Li Q Y, Feng Y, Lin Y N, Li M, Guo Q, Gu S Y, Liu J L, Zhang R F, Wan H Y
Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China
Department of Respiratory Medicine, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Genet Mol Res. 2014 Oct 20;13(4):8489-501. doi: 10.4238/2014.October.20.25.
Obstructive sleep apnea (OSA) is an independent risk factor for cardiovascular diseases such as systemic arterial hypertension, ischemic heart disease, stroke, heart failure, atrial fibrillation, and cardiac sudden death. The pathogenesis of cardiovascular disease in OSA is thought to be induced primarily by chronic intermittent hypoxia (CIH), a specific pattern of change in oxygenation during sleep. However, the underlying mechanisms of CIH-induced vasculature injury and gender differences are not well documented. The iTRAQ Quantitative Proteomic method enables analysis of a number of different proteins among several groups. Thus, we explored gender differences in protein expression in the vascular walls of mice exposed to CIH. C57BL/6J mice of each gender were exposed to CIH with a fractional inspired O2 (FiO2) nadir of 5% or control, with a treatment time of 8 h/day for 28 days. Differential proteins related to CIH-induced vascular injury between genders were identified using iTRAQ proteomic technology. A total of 163 proteins were identified, of which 34 showed significant differences between genders, which may correlate with vascular injury by CIH. Twenty up-regulated proteins and 14 downregulated proteins were observed in female mice compared with male mice. We identified different vascular proteins expressed under CIH between genders, suggesting that these proteins may be biomarkers of vascular injury by CIH.
阻塞性睡眠呼吸暂停（OSA）是心血管疾病的独立危险因素，如系统性动脉高血压、缺血性心脏病、中风、心力衰竭、心房颤动和心源性猝死。OSA中心血管疾病的发病机制被认为主要由慢性间歇性缺氧（CIH）引起，CIH是睡眠期间氧合的一种特定变化模式。然而，CIH诱导血管损伤的潜在机制以及性别差异尚未得到充分记录。iTRAQ定量蛋白质组学方法能够分析多组中的多种不同蛋白质。因此，我们探讨了暴露于CIH的小鼠血管壁中蛋白质表达的性别差异。将每种性别的C57BL/6J小鼠暴露于最低吸入氧分数（FiO2）为5%的CIH环境或作为对照，治疗时间为每天8小时，持续28天。使用iTRAQ蛋白质组学技术鉴定了与CIH诱导的性别间血管损伤相关的差异蛋白质。共鉴定出163种蛋白质，其中34种在性别间存在显著差异，这可能与CIH引起的血管损伤相关。与雄性小鼠相比，在雌性小鼠中观察到20种上调蛋白质和14种下调蛋白质。我们鉴定了性别间在CIH条件下表达的不同血管蛋白质，表明这些蛋白质可能是CIH诱导血管损伤的生物标志物。
