Pfeiffer Martin J, Taher Leila, Drexler Hannes, Suzuki Yutaka, Makałowski Wojciech, Schwarzer Caroline, Wang Bingyuan, Fuellen Georg, Boiani Michele
Max Planck Institute for Molecular Biomedicine, Münster, Germany; Institute for Biostatistics and Informatics in Medicine and Ageing Research, Rostock University Medical Center, Rostock, Germany.
Proteomics. 2015 Feb;15(4):675-87. doi: 10.1002/pmic.201400334. Epub 2015 Jan 3.
Current models of early mouse development assign roles to stochastic processes and epigenetic regulation, which are considered to be as influential as the genetic differences that exist between strains of the species Mus musculus. The aim of this study was to test whether mouse oocytes vary from each other in the abundance of gene products that could influence, prime, or even predetermine developmental trajectories and features of derivative embryos. Using the paradigm of inbred mouse strains, we quantified 2010 protein groups (SILAC LC-MS/MS) and 15205 transcripts (RNA deep sequencing) present simultaneously in oocytes of four strains tested (129/Sv, C57Bl/6J, C3H/HeN, DBA/2J). Oocytes differed according to donor strain in the abundance of catalytic and regulatory proteins, as confirmed for a subset (bromodomain adjacent to zinc finger domain, 1B [BAZ1B], heme oxygenase 1 [HMOX1], estrogen related receptor, beta [ESRRB]) via immunofluorescence in situ. Given a Pearson's r correlation coefficient of 0.18-0.20, the abundance of oocytic proteins could not be predicted from that of cognate mRNAs. Our results document that a prerequisite to generate embryo diversity, namely the different abundances of maternal proteins in oocytes, can be studied in the model of inbred mouse strains. Thus, we highlight the importance of proteomic quantifications in modern embryology. All MS data have been deposited in the ProteomeXchange with identifier PXD001059 (http://proteomecentral.proteomexchange.org/dataset/PXD001059).
当前早期小鼠发育模型认为随机过程和表观遗传调控发挥着重要作用，其影响力与小家鼠品系间存在的基因差异相当。本研究旨在测试小鼠卵母细胞中可能影响、启动甚至预先决定衍生胚胎发育轨迹和特征的基因产物丰度是否存在差异。利用近交系小鼠品系这一范例，我们对所测试的四个品系（129/Sv、C57Bl/6J、C3H/HeN、DBA/2J）的卵母细胞中同时存在的2010个蛋白质组（稳定同位素标记氨基酸细胞培养结合液相色谱-串联质谱法）和15205个转录本（RNA深度测序）进行了定量分析。卵母细胞中催化蛋白和调节蛋白的丰度因供体品系而异，通过免疫荧光原位法对一个子集（锌指结构域相邻的溴结构域1B [BAZ1B]、血红素加氧酶1 [HMOX1]、雌激素相关受体β [ESRRB]）进行检测得以证实。鉴于皮尔逊相关系数为0.18 - 0.20，无法根据同源mRNA的丰度预测卵母细胞蛋白的丰度。我们的研究结果表明，在近交系小鼠品系模型中可以研究产生胚胎多样性的一个先决条件，即卵母细胞中母体蛋白的不同丰度。因此，我们强调了蛋白质组定量分析在现代胚胎学中的重要性。所有质谱数据已存入蛋白质组交换库，标识符为PXD001059（http://proteomecentral.proteomexchange.org/dataset/PXD001059）。
