Institute for Biological Research "Siniša Stanković", University of Belgrade, Bulevar Despota Stefana 142, 11060, Belgrade, Serbia.
Food Funct. 2014 Dec;5(12):3296-303. doi: 10.1039/c4fo00819g.
Agrocybe aegerita (Brig.) Sing is a basidiomycete, white rot fungus. Antimicrobial activities and the antiqourum effect on Pseudomonas aeruginosa of an A. aegerita methanolic extract were investigated. The extract showed very good antimicrobial activity against all the tested microorganisms in a dose dependent manner. Effects of the Sub-MIC, MIC and 2MIC of the A. aegerita methanolic extract regulated the virulence factors in the quorum sensing (QS) test, as well as biofilm formation on P. aeruginosa. Sub-inhibitory and inhibitory concentrations of the extract demonstrated the reduction of virulence factors such as pyocyanin production, twitching and swimming motility. The biofilm forming capability of P. aeruginosa PAO1 was also reduced in a concentration-dependent manner. Furthermore, the chemical composition of the methanolic extract was determined considering its phenolic composition. The methanolic extract of A. aegerita can be a very good source of bioactive substances. This research is of great importance due to the prevalence of drug-resistant microorganisms.
柱状田头菇（Brig.）Sing 是一种担子菌，白腐真菌。研究了柱状田头菇甲醇提取物对铜绿假单胞菌的抗菌活性和抗老化作用。该提取物对所有测试的微生物均表现出良好的剂量依赖性抗菌活性。亚最小抑菌浓度（Sub-MIC）、最小抑菌浓度（MIC）和 2 倍 MIC 的 A. aegerita 甲醇提取物调节了群体感应（QS）试验中的毒力因子以及铜绿假单胞菌的生物膜形成。亚抑制和抑制浓度的提取物显示出减少毒力因子的作用，如绿脓菌素的产生、扭动和游泳运动。铜绿假单胞菌 PAO1 的生物膜形成能力也呈浓度依赖性降低。此外，还考虑了其酚类成分来确定甲醇提取物的化学成分。柱状田头菇的甲醇提取物可以是生物活性物质的很好来源。由于耐药微生物的流行，这项研究具有重要意义。
