Morita Ayako, Natsuhara Kazumi, Tomitsuka Eriko, Odani Shingo, Baba Jun, Tadokoro Kiyoshi, Igai Katsura, Greenhill Andrew R, Horwood Paul F, Soli Kevin W, Phuanukoonnon Suparat, Siba Peter M, Umezaki Masahiro
Department of Human Ecology, The University of Tokyo, Tokyo, 113-0033, Japan; Department of Health Promotion, Tokyo Medical and Dental University, Tokyo, 113-0034, Japan.
Am J Hum Biol. 2015 May-Jun;27(3):349-57. doi: 10.1002/ajhb.22647. Epub 2014 Nov 3.
The aim of this article was to develop a semi-quantitative food frequency questionnaire (FFQ) and evaluate its validity to estimate habitual protein intake, and investigate current dietary protein intakes of Papua New Guinea (PNG) Highlanders.
A 32-item FFQ was developed and tested among 135 healthy male and female volunteers. The FFQ-estimated daily total and animal protein intakes were compared with biomarkers and 3-day Weighed Food Records (WFR) by correlation analyses, Bland-Altman plot analyses and joint classification analyses.
The FFQ-estimated total protein intake significantly correlated with urinary nitrogen in the first morning void after adjusting urinary creatinine concentration (r = 0.28, P < 0.01) and the FFQ-estimated animal protein intake significantly correlated with the hair δ(15) N (Spearman's r = 0.34, P < 0.001). The limits of agreement were ±2.39 Z-score residuals for total protein intake and ±2.19 Z-score for animal protein intake, and intra-individual differences increased as protein intake increased. The classification into the same and adjacent quartiles was 66.0% for total protein intake and 73.6% for animal protein intake. Median daily total and animal protein intake estimates from the FFQ and the 3-day WFR showed a good agreement with differences of 0.2 and 4.9 g, respectively. None of the studied communities in the PNG Highlands met the biologically required protein intake; although the community closer to an urban center showed higher protein intake than the more remote communities.
The newly developed 32-item FFQ for PNG Highlanders is applicable for evaluation of protein intake at the individual level. Am. J. Hum. Biol. 27:349-357, 2015. © 2014 Wiley Periodicals, Inc.
本文旨在开发一种半定量食物频率问卷（FFQ），评估其估算习惯性蛋白质摄入量的有效性，并调查巴布亚新几内亚（PNG）高地居民当前的膳食蛋白质摄入量。
开发了一份包含32个条目的FFQ，并在135名健康的男性和女性志愿者中进行测试。通过相关性分析、布兰德-奥特曼图分析和联合分类分析，将FFQ估算的每日总蛋白质摄入量和动物蛋白摄入量与生物标志物及3天称重食物记录（WFR）进行比较。
在调整尿肌酐浓度后，FFQ估算的总蛋白质摄入量与晨尿中的尿氮显著相关（r = 0.28，P < 0.01），FFQ估算的动物蛋白摄入量与毛发δ(15)N显著相关（斯皮尔曼r = 0.34，P < 0.001）。总蛋白质摄入量的一致性界限为±2.39个Z分数残差，动物蛋白摄入量为±2.19个Z分数，个体内差异随着蛋白质摄入量的增加而增大。总蛋白质摄入量和动物蛋白摄入量在相同和相邻四分位数中的分类分别为66.0%和73.6%。FFQ和3天WFR估算的每日总蛋白质摄入量和动物蛋白摄入量中位数显示出良好的一致性，差异分别为0.2克和4.9克。PNG高地的所有研究社区均未达到生物学所需的蛋白质摄入量；尽管靠近城市中心的社区比更偏远的社区蛋白质摄入量更高。
新开发的针对PNG高地居民的32项FFQ适用于个体水平的蛋白质摄入量评估。《美国人类生物学杂志》27:349 - 357，2015年。© 2014威利期刊公司
