实验性口腔癌发生过程中潜在恶性病变和癌组织中的血管生成：仓鼠颊囊的初步研究
Angiogenesis in potentially malignant lesions and carcinomas during experimental oral carcinogenesis: a preliminary study in the hamster cheek pouch.
作者信息
Aromando Romina F, Raimondi Ana R, Pérez Miguel A, Trivillin Veronica A, Schwint Amanda E, Itoiz Maria E
机构信息
Department of Oral Pathology, Faculty of Dentistry, University of Buenos Aires, Buenos Aires, Argentina
Research Area Institute of Oncology Angel H. Roffo, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
出版信息
Anticancer Res. 2014 Nov;34(11):6381-8.
AIM
To evaluate vascular morphology and density, angiogenic switch activation, vascular endothelial growth factor (VEGF) expression, and endothelial cell (EC) proliferation in the hamster cheek pouch (HCP) model of oral cancer.
MATERIALS AND METHODS
Immunohistochemical detection of factor VIII, 5'-Bromo-2'-Deoxyuridine (BrdU) and VEGF was performed in pre-malignant and tumoral tissues.
RESULTS
Activation of angiogenesis was detected adjacent to epithelial dysplasia. Vascularized area and perimeter (p<0.001) increased in dysplasias and tumors. Tumor blood vessels exhibited an enhanced vascular compression (p<0.001) and structural alterations. EC proliferation was similar in dysplasias and carcinomas. An increase in vascular density, EC proliferation and VEGF expression was found in potentially malignant tissues but not in carcinomas.
CONCLUSION
The angiogenic switch occurs in the dysplastic stage preceding tumor development in the HCP model of oral cancer. In potentially malignant tissues, increased VEGF expression favors EC proliferation and an increase in vascular density. Conversely, in tumors, VEGF is no longer of pivotal importance.
目的
评估口腔癌金黄地鼠颊囊（HCP）模型中的血管形态与密度、血管生成开关激活、血管内皮生长因子（VEGF）表达以及内皮细胞（EC）增殖情况。
材料与方法
对癌前组织和肿瘤组织进行因子VIII、5'-溴-2'-脱氧尿苷（BrdU）和VEGF的免疫组织化学检测。
结果
在上皮发育异常附近检测到血管生成激活。发育异常和肿瘤中的血管化面积和周长增加（p<0.001）。肿瘤血管表现出增强的血管受压（p<0.001）和结构改变。发育异常和癌中的EC增殖相似。在潜在恶性组织中发现血管密度、EC增殖和VEGF表达增加，但在癌中未发现。
结论
在口腔癌HCP模型中，血管生成开关发生在肿瘤发生之前的发育异常阶段。在潜在恶性组织中，VEGF表达增加有利于EC增殖和血管密度增加。相反，在肿瘤中，VEGF不再具有关键重要性。
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