Garcia C, Rosén A, Kimby E, Aguilar-Santelises M, Jondal M, Bjorkholm M, Holm G, Mellstedt H
Immunological Research Laboratory, Karolinska Hospital, Stockholm, Sweden.
Leuk Res. 1989;13(1):31-7. doi: 10.1016/0145-2126(89)90028-3.
The surface marker phenotype of lymphocytes derived from 12 patients with B-CLL was compared to that of lymphocytes from 10 patients with an other monoclonal but clinical benign form of B-cell proliferative disorder termed monoclonal B-cell lymphocytosis of undetermined significance (B-MLUS). A panel of well characterized monoclonal antibodies was used for the surface marker determinations. The mean total number of B cells (CD20) was 8.5 x 10(9)/1 in B-MLUS as compared to 44 x 10(9)/1 in B-CLL (p less than 0.001). B-CLL had a greater imbalance in T-cell subpopulations than B-MLUS and healthy controls. Total numbers of CD3+, CD8+ cells as well as cells expressing the NK-related antigens (CD16, Leu-7) and IL-2 receptor (CD25) bearing lymphocytes were statistically significant higher in B-CLL than in B-MLUS. Analyses of B-cell enriched populations showed that B-CLL represented B cells of an early maturation stage, whereas B cells from B-MLUS were more mature as judged by the loss of the CD21 surface marker. A larger fraction of B cells in B-CLL compared to B-MLUS exhibited a higher activation stage as revealed by the expression of the CD21, CD25 and CD35 structures as well as the FMC7 antigen.
将12例B细胞慢性淋巴细胞白血病（B-CLL）患者的淋巴细胞表面标志物表型，与10例患有另一种单克隆但临床良性的B细胞增殖性疾病（称为意义未明的单克隆B细胞淋巴细胞增多症，B-MLUS）患者的淋巴细胞表面标志物表型进行了比较。使用一组经过充分表征的单克隆抗体进行表面标志物测定。B-MLUS中B细胞（CD20）的平均总数为8.5×10⁹/升，而B-CLL中为44×10⁹/升（p<0.001）。与B-MLUS和健康对照相比，B-CLL在T细胞亚群中的失衡更为严重。B-CLL中CD3⁺、CD8⁺细胞以及表达NK相关抗原（CD16、Leu-7）和白细胞介素-2受体（CD25）的淋巴细胞总数在统计学上显著高于B-MLUS。对富含B细胞的群体分析表明，B-CLL代表早期成熟阶段的B细胞，而根据CD21表面标志物的缺失判断，B-MLUS的B细胞更为成熟。与B-MLUS相比，B-CLL中更大比例的B细胞表现出更高的激活阶段，这通过CD21、CD25和CD35结构以及FMC7抗原的表达得以揭示。
