Kimby E, Mellstedt H, Nilsson B, Björkholm M, Holm G
Department of Medicine, Danderyd Hospital, Sweden.
Leukemia. 1989 Jul;3(7):501-4.
T and NK cell blood subpopulations were determined in 33 patients with B-CLL and in 14 patients with B-MLUS by two-color immunofluorescence. CLL patients had significantly higher total numbers of Leu-7+ and CD8+ cells and lower numbers of CD16+/Leu-7- cells as well as a higher Leu-7/CD16 ratio and a lower CD4/CD8 ratio than MLUS patients and control donors. Moreover, MLUS patients exhibited a significantly lower Leu-7/CD16 ratio as well as a higher frequency of CD16+/Leu-7- cells than healthy donors. These results suggest that B-CLL patients have higher numbers of circulating immature NK cells compared to B-MLUS, while B-MLUS patients have a larger proportion of NK cells with a high lytic capability as compared to both CLL and normal controls. The imbalance between CD4+ and CD8+ cells was prominent in CLL with a low CD4/CD8 ratio, but within the upper normal range in MLUS. Differences in immunoregulatory cell subpopulations between B-CLL and B-MLUS might therefore contribute to the different clinical behavior of these two disorders.
通过双色免疫荧光法测定了33例B细胞慢性淋巴细胞白血病（B-CLL）患者和14例B细胞单克隆淋巴细胞增多症（B-MLUS）患者的T细胞和NK细胞血液亚群。与B-MLUS患者及对照供者相比，CLL患者的Leu-7+和CD8+细胞总数显著更高，CD16+/Leu-7-细胞数量更低，Leu-7/CD16比值更高，CD4/CD8比值更低。此外，与健康供者相比，B-MLUS患者的Leu-7/CD16比值显著更低，CD16+/Leu-7-细胞频率更高。这些结果表明，与B-MLUS相比，B-CLL患者循环中未成熟NK细胞数量更多，而与CLL和正常对照相比，B-MLUS患者具有高裂解能力的NK细胞比例更大。CLL患者中CD4+和CD8+细胞之间的失衡较为突出，CD4/CD8比值较低，但在B-MLUS中处于正常上限范围内。因此，B-CLL和B-MLUS之间免疫调节细胞亚群的差异可能导致这两种疾病不同的临床行为。
