Enrietto P J
Department of Microbiology, State University of New York at Stony Brook 11794.
Virology. 1989 Feb;168(2):256-66. doi: 10.1016/0042-6822(89)90265-1.
In order to characterize the role of the carboxy terminus of the viral myc protein in the transformation of avian fibroblasts and macrophages, several Bal31 deletion mutants were created which removed varying portions of the carboxy terminus of the myc protein. Only one such mutant, S90.9, which had lost nine amino acids of myc retained biological activity when tested in macrophages and fibroblasts. This mutant transformed avian macrophages in a manner similar to that of wild-type, but appeared to be partially transformation defective in fibroblasts. Chicken embryo fibroblast cultures infected with S90.9 exhibited an intermediate phenotype morphologically when compared to wild-type-infected cells. When tested for growth in soft agar, the presence or absence of actin cables and fibronectin on the cell surface, and growth rate, S90.9-infected cells showed intermediate behavior when compared to wild-type or helper virus-infected fibroblasts. These experiments suggest that the carboxy terminus of the myc protein, which is highly basic in nature, is involved in the transformation of avian fibroblasts.
为了阐明病毒myc蛋白的羧基末端在禽成纤维细胞和巨噬细胞转化中的作用，构建了几个Bal31缺失突变体，这些突变体去除了myc蛋白羧基末端的不同部分。在巨噬细胞和成纤维细胞中进行测试时，只有一个这样的突变体S90.9（其myc蛋白缺失了九个氨基酸）保留了生物学活性。该突变体转化禽巨噬细胞的方式与野生型相似，但在成纤维细胞中似乎存在部分转化缺陷。与野生型感染的细胞相比，感染S90.9的鸡胚成纤维细胞培养物在形态上表现出中间表型。在软琼脂中测试生长情况、细胞表面肌动蛋白丝和纤连蛋白的有无以及生长速率时，与野生型或辅助病毒感染的成纤维细胞相比，感染S90.9的细胞表现出中间行为。这些实验表明，本质上高度碱性的myc蛋白羧基末端参与了禽成纤维细胞的转化。
