Yokota T, Takahashi T, Yamaguchi T, Sawai K
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
Am J Clin Pathol. 1989 Feb;91(2):187-90. doi: 10.1093/ajcp/91.2.187.
The authors used the monoclonal antibody (MAb) S202 as an adjunct for detection of scirrhous gastric carcinoma cells in cytomorphologic preparations of the resected stomach. Twenty-six samples of scirrhous gastric cancer were stained by the avidin-biotin complex technique on fixed frozen sections. MAb S202 was strongly reactive on all sections. In 12 cases, samples obtained from surgical margins were examined by a rapid immunostaining method. In one case, conventional cytologic findings were equivocal, whereas by the rapid immunostaining method single cells were stained darkly, indicating malignancy. Thus, immunohistochemical analysis using MAb S202 should be carried out to determine the limit of invasive carcinoma at the time of surgery.
作者使用单克隆抗体（MAb）S202辅助检测切除胃的细胞形态学标本中的硬癌胃癌细胞。对26例硬癌胃癌标本进行固定冰冻切片，采用抗生物素蛋白-生物素复合物技术染色。MAb S202在所有切片上均呈强反应性。12例中，采用快速免疫染色法检查手术切缘标本。1例中，传统细胞学检查结果不明确，但快速免疫染色法显示单个细胞被深染，提示为恶性。因此，手术时应采用MAb S202进行免疫组化分析以确定浸润癌的边界。
