Yokota T, Masuko T, Yamaguchi T, Kitamura K, Takahashi T, Hashimoto Y
First Department of Surgery, Kyoto Prefectural, University of Medicine, Japan.
Jpn J Surg. 1989 Jul;19(4):452-8. doi: 10.1007/BF02471627.
A monoclonal antibody (MAb) S202, with an IgG1 isotype, that reacted strongly with the scirrhous gastric carcinoma cell line MK-01 was established. MAb S202 reacted with the colonic cancer cell line, SW1116, and the pancreatic cancer cell line, PK-1, when tested by indirect immunofluorescence. The S202 reactive antigen was expressed in the majority of acetone-fixed fresh frozen cancer tissues. Eighty to 100 per cent of the paraffin-embedded sections of stomach, colon and pancreatic adenocarcinoma were positive for the S202 antigen, with diffuse cytoplasmic staining, whereas esophageal and breast cancers demonstrated markedly less immunostaining. Supplemented serum-free medium collected from 7 day old tumor cell cultures were assayed for the presence of antibody-defined antigens. Antigens detected by MAb S202 were released by the cell lines SW1116 and PK-1. The binding of MAb S202 to the colonic adenocarcinoma sections was reduced after treatment with sodium periodate which suggests that respective antigenic determinants are of carbohydrate nature without sialic acid residues.
建立了一种单克隆抗体（MAb）S202，其亚型为IgG1，能与硬癌胃癌细胞系MK - 01强烈反应。通过间接免疫荧光检测时，MAb S202与结肠癌细胞系SW1116和胰腺癌细胞系PK - 1发生反应。S202反应性抗原在大多数丙酮固定的新鲜冷冻癌组织中表达。胃、结肠和胰腺腺癌石蜡包埋切片的80%至100%对S202抗原呈阳性，呈弥漫性细胞质染色，而食管癌和乳腺癌的免疫染色明显较少。对从7日龄肿瘤细胞培养物中收集的无血清培养基补充液进行抗体定义抗原检测。MAb S202检测到的抗原由细胞系SW1116和PK - 1释放。高碘酸钠处理后，MAb S202与结肠腺癌切片的结合减少，这表明相应的抗原决定簇具有碳水化合物性质，且没有唾液酸残基。
