Wyand M S, Ringler D J, Naidu Y M, Mattmuller M, Chalifoux L V, Sehgal P K, Daniel M D, Desrosiers R C, King N W
Harvard Medical School, New England Regional Primate Research Center, Southborough, MA 01772.
Am J Pathol. 1989 Feb;134(2):385-93.
Lymph nodes and spleens were collected at autopsy and by biopsy from 29 rhesus monkeys infected with simian immunodeficiency virus (SIV). Lymph nodes were classified morphologically into stages of follicular hyperplasia, follicular involution, follicular depletion with normal or expanded paracortices, follicular and paracortical depletion, granulomatous lymphadenitis, or normal. The distribution of SIV RNA was determined by in situ hybridization using a nick translated, 35S labeled, SIVmac DNA probe. Numbers of SIV-infected cells were rare during follicular hyperplasia, numerous during follicular and paracortical expansion, and rare during follicular and paracortical depletion. The splenic morphology reflected that of the lymph nodes; however, the numbers of SIV-positive cells were uniformly lower. SIV RNA was frequently restricted to a single nucleus within multinucleate syncytial cells in two cases of granulomatous lymphadenitis. These results, combined with those of a previous study, provide evidence for antigen trapping in SIV-infected hyperplastic lymph nodes and for widespread viral infection of macrophages and lymphocytes during paracortical expansion.
在尸检时以及通过活检从29只感染了猴免疫缺陷病毒（SIV）的恒河猴身上采集了淋巴结和脾脏。淋巴结在形态学上被分类为滤泡增生、滤泡退化、滤泡耗竭伴正常或扩大的副皮质、滤泡和副皮质耗竭、肉芽肿性淋巴结炎或正常阶段。使用缺口平移、35S标记的SIVmac DNA探针通过原位杂交确定SIV RNA的分布。在滤泡增生期间，感染SIV的细胞数量很少，在滤泡和副皮质扩张期间数量众多，而在滤泡和副皮质耗竭期间则很少。脾脏形态反映了淋巴结的形态；然而，SIV阳性细胞的数量始终较低。在两例肉芽肿性淋巴结炎中，SIV RNA经常局限于多核合胞体细胞内的单个细胞核中。这些结果与先前一项研究的结果相结合，为SIV感染的增生性淋巴结中的抗原捕获以及副皮质扩张期间巨噬细胞和淋巴细胞的广泛病毒感染提供了证据。
