Fuki I V, Preobrazhensky S N, Bushmakina N G, Menschikov G B, Repin V S, Karpov R S
Laboratory of Cell and Tissue Culture, USSR Cardiology Research Center, Moscow.
Biochim Biophys Acta. 1989 Feb 6;1001(2):235-8. doi: 10.1016/0005-2760(89)90153-7.
Human hepatoma HepG2 cells were used to study the effects of cholesterol loading and depletion on apolipoprotein B (apoB) secretion and low-density lipoprotein (LDL) receptor activity. Exposure of HepG2 cells to cholesterol and oleic acid, which elevated intracellular cholesterol levels, stimulated apoB secretion and reduced receptor-mediated uptake of LDL, whereas recombinant complexes of apolipoprotein A-I with dimyristoylphosphatidylcholine, which depleted the cellular cholesterol pool, inhibited apoB secretion and up-regulated LDL receptors. Significant negative correlation (r = -0.92, P less than 0.001) between the levels of apoB secretion and LDL uptake was found. These data suggest that the cholesterol content of the cells may induce concomitant changes in apoB secretion and LDL receptor activity.
用人肝癌HepG2细胞研究胆固醇加载和消耗对载脂蛋白B（apoB）分泌及低密度脂蛋白（LDL）受体活性的影响。将HepG2细胞暴露于胆固醇和油酸中可提高细胞内胆固醇水平，刺激apoB分泌并减少受体介导的LDL摄取，而载脂蛋白A-I与二肉豆蔻酰磷脂酰胆碱的重组复合物可消耗细胞胆固醇池，抑制apoB分泌并上调LDL受体。发现apoB分泌水平与LDL摄取之间存在显著负相关（r = -0.92，P < 0.001）。这些数据表明，细胞内胆固醇含量可能会引起apoB分泌和LDL受体活性的相应变化。
