• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用荧光素酶报告基因测定法，检测双酚S、聚醚砜及其在大鼠肝脏S9组分作用下产生的代谢产物对MVLN细胞的雌激素活性。

Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.

作者信息

Kang Jae Soon, Choi Jin-Soo, Kim Woo-Keun, Lee Yong-Ju, Park June-Woo

机构信息

Gyeongnam Department of Environmental Toxicology and Chemistry, Korea Institute of Toxicology, Jin-Ju, Gyeongnam, Republic of Korea.

出版信息

Reprod Biol Endocrinol. 2014 Nov 4;12:102. doi: 10.1186/1477-7827-12-102.

DOI:10.1186/1477-7827-12-102
PMID:25371027
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4232735/
Abstract

BACKGROUND

Bisphenol A (BPA) is an applied chemical that is used in many industrial fields and is a potential endocrine disruption chemical (EDC) that is found in the environment. Bisphenol S (BPS) and polyethersulfone (PES) have been suggested as putative BPA alternatives. In this study, the estrogenic potency induced by the binding of 17-beta-estradiol (E2), BPA, BPS, PES and their metabolites formed by the rat liver S9 fraction to the human estrogen receptor (ER) was estimated.

METHODS

We used an in vitro bioassay based on the luciferase reporter assay in MVLN cells to evaluate the estrogenic activity of 17-beta-estradiol (E2), BPA, BPS, PES (E2: 0.001 to 0.3 nM; BPA, BPS and PES: 0.0001 to 5 microM) and their metabolites (E2: 0.05 microM; BPA, BPS and PES: 0.1 mM) according to incubation times (0, 20 and 40 min). After chemical treatment to MVLN cells for 72 hrs, and the cell viability and luciferase intensity induced were estimated, from which the estrogenic activity of the chemicals tested was evaluated.

RESULTS

BPA and BPS induced estrogenic activity whereas PES did not show any estrogenic activity in the concentrations tested. In an in vitro assay of metabolites, BPA metabolites displayed comparable estrogenic activity with BPA and metabolites of both BPS and PES showed increasing estrogenic activity.

CONCLUSIONS

The results suggest that the metabolites of BPS and PES have estrogenic potential and the need for the assessment of both chemicals and their metabolites in other EDC evaluation studies. The estrogenic potency of PES and its metabolites is the first report in our best knowledge.

摘要

背景

双酚A（BPA）是一种应用于许多工业领域的化学物质，是一种在环境中被发现的潜在内分泌干扰化学物质（EDC）。双酚S（BPS）和聚醚砜（PES）已被提议作为BPA的替代物。在本研究中，评估了17-β-雌二醇（E2）、BPA、BPS、PES及其由大鼠肝脏S9组分形成的代谢产物与人类雌激素受体（ER）结合所诱导的雌激素活性。

方法

我们使用基于MVLN细胞中荧光素酶报告基因检测的体外生物测定法，根据孵育时间（0、20和40分钟）评估17-β-雌二醇（E2）、BPA、BPS、PES（E2：0.001至0.3 nM；BPA、BPS和PES：0.0001至5 μM）及其代谢产物（E2：0.05 μM；BPA、BPS和PES：0.1 mM）的雌激素活性。在对MVLN细胞进行化学处理72小时后，估计细胞活力和诱导的荧光素酶强度，据此评估所测试化学物质的雌激素活性。

结果

在测试浓度下，BPA和BPS诱导了雌激素活性，而PES未显示任何雌激素活性。在代谢产物的体外测定中，BPA代谢产物表现出与BPA相当的雌激素活性，BPS和PES的代谢产物均表现出增加的雌激素活性。

结论

结果表明，BPS和PES的代谢产物具有雌激素潜力，并且在其他EDC评估研究中有必要对这两种化学物质及其代谢产物进行评估。据我们所知，PES及其代谢产物的雌激素活性是首次报道。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/e07c96924679/12958_2014_1271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/826388636e0f/12958_2014_1271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/84f084371cb7/12958_2014_1271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/3b68ebe3e7a3/12958_2014_1271_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/7d41c3b5f727/12958_2014_1271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/e07c96924679/12958_2014_1271_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/826388636e0f/12958_2014_1271_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/84f084371cb7/12958_2014_1271_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/3b68ebe3e7a3/12958_2014_1271_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/7d41c3b5f727/12958_2014_1271_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/eb60/4232735/e07c96924679/12958_2014_1271_Fig5_HTML.jpg

相似文献

1
Estrogenic potency of bisphenol S, polyethersulfone and their metabolites generated by the rat liver S9 fractions on a MVLN cell using a luciferase reporter gene assay.使用荧光素酶报告基因测定法，检测双酚S、聚醚砜及其在大鼠肝脏S9组分作用下产生的代谢产物对MVLN细胞的雌激素活性。
Reprod Biol Endocrinol. 2014 Nov 4;12:102. doi: 10.1186/1477-7827-12-102.
2
Potent estrogenic metabolites of bisphenol A and bisphenol B formed by rat liver S9 fraction: their structures and estrogenic potency.大鼠肝脏S9组分形成的双酚A和双酚B的强效雌激素代谢物：它们的结构和雌激素活性。
Toxicol Sci. 2004 Mar;78(1):50-9. doi: 10.1093/toxsci/kfh047. Epub 2003 Dec 22.
3
In vitro and in vivo estrogenic activity of BPA, BPF and BPS in zebrafish-specific assays.在鱼类特定试验中，BPA、BPF 和 BPS 的体外和体内雌激素活性。
Ecotoxicol Environ Saf. 2017 Aug;142:150-156. doi: 10.1016/j.ecoenv.2017.04.009. Epub 2017 Apr 11.
4
In vitro study on the agonistic and antagonistic activities of bisphenol-S and other bisphenol-A congeners and derivatives via nuclear receptors.通过核受体对双酚 S 及其它双酚 A 同系物和衍生物的激动和拮抗活性的体外研究。
Toxicol Appl Pharmacol. 2013 Oct 1;272(1):127-36. doi: 10.1016/j.taap.2013.05.015. Epub 2013 May 25.
5
Ipso substitution of bisphenol A catalyzed by microsomal cytochrome P450 and enhancement of estrogenic activity.微粒体细胞色素 P450 催化的双酚 A 的同系物取代及其雌激素活性的增强。
Toxicol Lett. 2011 May 30;203(1):92-5. doi: 10.1016/j.toxlet.2011.03.010. Epub 2011 Mar 23.
6
Influence of metabolism on endocrine activities of bisphenol S.代谢对双酚S内分泌活性的影响。
Chemosphere. 2016 Aug;157:152-9. doi: 10.1016/j.chemosphere.2016.05.027. Epub 2016 May 21.
7
Metabolic activation of bisphenol A by rat liver S9 fraction.双酚A经大鼠肝脏S9组分的代谢活化作用。
Toxicol Sci. 2001 Aug;62(2):221-7. doi: 10.1093/toxsci/62.2.221.
8
Additional effects of bisphenol A and paraben on the induction of calbindin-D(9K) and progesterone receptor via an estrogen receptor pathway in rat pituitary GH3 cells.双酚 A 和对羟基苯甲酸酯通过雌激素受体途径对大鼠垂体 GH3 细胞钙结合蛋白 D(9K)和孕激素受体诱导的额外作用。
J Physiol Pharmacol. 2012 Oct;63(5):445-55.
9
[Detection of estrogenic effects of nonylphenol and bisphenol A in vitro reporter gene-based assays].[基于体外报告基因检测法检测壬基酚和双酚A的雌激素效应]
Wei Sheng Yan Jiu. 2006 Jan;35(1):13-5.
10
Differential Biological Action, Coregulator Interactions, and Molecular Dynamic Analysis of Bisphenol A (BPA), BPAF, and BPS Ligand-ERα Complexes.双酚 A（BPA）、BPAF 和 BPS 配体-ERα 复合物的差异生物学作用、共调节剂相互作用及分子动力学分析。
Environ Health Perspect. 2018 Jan 31;126(1):017012. doi: 10.1289/EHP2505.

引用本文的文献

1
Nongenomic ERα-AMPK Signaling Regulates Sex-Dependent Bcrp Transport Activity at the Blood-Brain Barrier.非基因组 ERα-AMPK 信号调节血脑屏障上性别依赖性 Bcrp 转运活性。
Endocrinology. 2024 Jul 1;165(8). doi: 10.1210/endocr/bqae081.
2
Toxicological investigation of lilial.利拉香精的毒理学研究。
Sci Rep. 2023 Oct 28;13(1):18536. doi: 10.1038/s41598-023-45598-y.
3
Maternal perinatal exposure to bisphenol S induces an estrogenic like effect in glucose homeostasis in male offspring.母代围产期暴露于双酚 S 会在雄性后代的葡萄糖稳态中诱导出类雌激素效应。

本文引用的文献

1
Endocrine disruptors differentially target ATP-binding cassette transporters in the blood-testis barrier and affect Leydig cell testosterone secretion in vitro.内分泌干扰物在血睾屏障中差异靶向 ATP 结合盒转运蛋白，并影响体外莱迪希细胞的睾酮分泌。
Toxicol Sci. 2013 Dec;136(2):382-91. doi: 10.1093/toxsci/kft198. Epub 2013 Sep 6.
2
The effects of nanomaterials as endocrine disruptors.纳米材料作为内分泌干扰物的影响。
Int J Mol Sci. 2013 Aug 14;14(8):16732-801. doi: 10.3390/ijms140816732.
3
Bisphenol S disrupts estradiol-induced nongenomic signaling in a rat pituitary cell line: effects on cell functions.
Environ Toxicol. 2022 Sep;37(9):2189-2200. doi: 10.1002/tox.23585. Epub 2022 May 21.
4
A Data Mining Approach Reveals Chemicals Detected at Higher Levels in Non-Hispanic Black Women Target Preterm Birth Genes and Pathways.一种数据挖掘方法揭示了在非西班牙裔黑人体内水平较高的化学物质靶向早产相关基因和途径。
Reprod Sci. 2022 Jul;29(7):2001-2012. doi: 10.1007/s43032-022-00870-w. Epub 2022 Feb 2.
5
Exposure to New Emerging Bisphenols Among Young Children in Switzerland.瑞士幼儿接触新型双酚类物质的情况。
Int J Environ Res Public Health. 2020 Jul 3;17(13):4793. doi: 10.3390/ijerph17134793.
6
A Demonstration of the Uncertainty in Predicting the Estrogenic Activity of Individual Chemicals and Mixtures From an In Vitro Estrogen Receptor Transcriptional Activation Assay (T47D-KBluc) to the In Vivo Uterotrophic Assay Using Oral Exposure.从体外雌激素受体转录激活试验（T47D-KBluc）到口服暴露的体内子宫增重试验，预测单个化学品和混合物雌激素活性的不确定性的论证。
Toxicol Sci. 2016 Oct;153(2):382-95. doi: 10.1093/toxsci/kfw134. Epub 2016 Jul 29.
7
Estrogenic Effects of Several BPA Analogs in the Developing Zebrafish Brain.几种双酚A类似物对斑马鱼幼鱼大脑发育的雌激素效应
Front Neurosci. 2016 Mar 24;10:112. doi: 10.3389/fnins.2016.00112. eCollection 2016.
8
Estrogens and Male Lower Urinary Tract Dysfunction.雌激素与男性下尿路功能障碍
Curr Urol Rep. 2015 Sep;16(9):61. doi: 10.1007/s11934-015-0534-6.
双酚 S 破坏雌二醇诱导的大鼠垂体细胞系中的非基因组信号转导：对细胞功能的影响。
Environ Health Perspect. 2013 Mar;121(3):352-8. doi: 10.1289/ehp.1205826. Epub 2013 Jan 17.
4
Developmental exposure to bisphenol A modulates innate but not adaptive immune responses to influenza A virus infection.发育阶段接触双酚 A 会调节对甲型流感病毒感染的固有但非适应性免疫反应。
PLoS One. 2012;7(6):e38448. doi: 10.1371/journal.pone.0038448. Epub 2012 Jun 4.
5
Weak estrogenic transcriptional activities of Bisphenol A and Bisphenol S.双酚A和双酚S的弱雌激素转录活性。
Toxicol In Vitro. 2012 Aug;26(5):727-31. doi: 10.1016/j.tiv.2012.03.013. Epub 2012 Apr 5.
6
Administration, distribution, metabolism and elimination of polymer therapeutics.聚合物治疗药物的管理、分布、代谢和消除。
J Control Release. 2012 Jul 20;161(2):446-60. doi: 10.1016/j.jconrel.2011.12.021. Epub 2011 Dec 29.
7
Bisphenol A in thermal paper receipts: an opportunity for evidence-based prevention.热敏纸收据中的双酚A：基于证据的预防契机。
Environ Health Perspect. 2012 Jan;120(1):A14-5; author reply A15. doi: 10.1289/ehp.1104004.
8
Comparison of migration from polyethersulphone and polycarbonate baby bottles.聚醚砜和聚碳酸酯婴儿奶瓶中迁移物的比较。
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011 Dec;28(12):1763-8. doi: 10.1080/19440049.2011.604644. Epub 2011 Oct 11.
9
Estrogenic activity of alkylphenols, bisphenol S, and their chlorinated derivatives using a GFP expression system.利用 GFP 表达系统评估烷基酚、双酚 S 及其氯化衍生物的雌激素活性。
Environ Toxicol Pharmacol. 2005 Jan;19(1):121-30. doi: 10.1016/j.etap.2004.05.009.
10
Bisphenol A increases mammary cancer risk in two distinct mouse models of breast cancer.双酚 A 增加了两种不同乳腺癌小鼠模型的乳腺癌风险。
Biol Reprod. 2011 Sep;85(3):490-7. doi: 10.1095/biolreprod.110.090431. Epub 2011 Jun 2.