Schrag J D, Prasad B V, Rixon F J, Chiu W
University of Arizona, Department of Biochemistry, Tucson 85721.
Cell. 1989 Feb 24;56(4):651-60. doi: 10.1016/0092-8674(89)90587-4.
The three-dimensional structures of full and empty capsids of HSV1 were determined by computer analysis of low dose cryo-electron images of ice embedded capsids. The full capsid structure is organized into outer, intermediate, and inner structural layers. The empty capsid structure has only one layer which is indistinguishable from the outer layer of the full capsids. This layer is arranged according to T = 16 icosahedral symmetry. The intermediate layer of full capsids appears to lie on a T = 4 icosahedral lattice. The genomic DNA is located inside the T = 4 shell and is the component of the innermost layer of the full capsids. The outer and intermediate layers interact in such a way that the channels along their icosahedral two-fold axis coincide and form a direct pathway between the DNA and the environment outside the capsid.
通过对冰包埋衣壳的低剂量冷冻电子图像进行计算机分析，确定了单纯疱疹病毒1型（HSV1）完整衣壳和空衣壳的三维结构。完整衣壳结构由外层、中层和内层结构层组成。空衣壳结构只有一层，与完整衣壳的外层无法区分。这一层按照T = 16二十面体对称排列。完整衣壳的中层似乎位于T = 4二十面体晶格上。基因组DNA位于T = 4壳内部，是完整衣壳最内层的组成部分。外层和中层相互作用，使得沿其二十面体二重轴的通道重合，形成了DNA与衣壳外部环境之间的直接通路。
