Zhdanov Vladimir P
Section of Biological Physics, Department of Applied Physics, Chalmers University of Technology, S-41296 Göteborg, Sweden and Boreskov Institute of Catalysis, Russian Academy of Sciences, Novosibirsk 630090, Russia.
Phys Rev E Stat Nonlin Soft Matter Phys. 2014 Oct;90(4):042721. doi: 10.1103/PhysRevE.90.042721. Epub 2014 Oct 27.
The available kinetic models of assembly of viral protein capsids are focused primarily on the situations in vitro where the amount of protein is fixed. In vivo, however, the viral protein synthesis and capsid assembly occur under transient conditions in parallel with viral genome replication. Herein, a kinetic model describing the latter case of capsid assembly is proposed with emphasis on the period corresponding to the initial stage of viral genome replication. The analysis is aimed at small icosahedral capsids. With biologically reasonable values of model parameters, the model predicts rapid exponential growth of the populations of monomers and fully assembled capsids during the transient period of genome replication. Under the subsequent steady-state conditions with respect to replication, the monomer population is predicted to be nearly constant while the number of fully assembled capsids increases linearly. The kinetics of capsid disassembly, described briefly as well under conditions of negligible monomer concentration, exhibit a short induction period when the number of proteins in a capsid is only slightly smaller than in the beginning, followed by more rapid protein detachment. According to calculations, the latter kinetics may strongly depend on protein degradation.
现有的病毒蛋白衣壳组装动力学模型主要聚焦于体外蛋白质数量固定的情况。然而，在体内，病毒蛋白合成和衣壳组装是在与病毒基因组复制并行的瞬态条件下发生的。在此，提出了一个描述衣壳组装后一种情况的动力学模型，重点关注与病毒基因组复制初始阶段相对应的时期。该分析针对的是小型二十面体衣壳。利用生物学上合理的模型参数值，该模型预测在基因组复制的瞬态期间，单体和完全组装衣壳的群体将快速指数增长。在随后关于复制的稳态条件下，预测单体群体几乎恒定，而完全组装衣壳的数量呈线性增加。在单体浓度可忽略不计的条件下简要描述的衣壳解聚动力学，当衣壳中的蛋白质数量仅略小于开始时，会有一个短的诱导期，随后蛋白质脱离更快。根据计算，后者的动力学可能强烈依赖于蛋白质降解。
