Wu Pinggu, Cai Chenggang, Yang Dajin, Wang Liyuan, Zhou Yan, Shen Xianghong, Ma Bingjie, Tang Jun
Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
J Sep Sci. 2015 Jan;38(2):254-9. doi: 10.1002/jssc.201400718. Epub 2014 Dec 2.
A detection method for 19 kinds of phthalic acid ester compounds analyzed by n-hexane/ether/acetonitrile 1:7:8 v/v/v mixed solvent extraction, quick, easy, cheap, effective, rugged, and safe purification and internal standard method of quantitative gas chromatography with mass spectrometry was established. This method can effectively remove interfering materials, such as lipids, fatty acids, and pigments, from dairy products. The 19 kinds of phthalic acid ester compounds were within a 0.025-0.2 mg/kg range, the recovery rate was 65.2-125.7%, relative standard deviation was 7.9-15.4% (n = 6), and the limit of detection was 0.005-0.02 mg/kg. Concentrations of the 19 kinds of phthalic acid ester compounds ranged between 0.01 and 0.12 mg/kg in ten dairy materials and 20 dairy products. The established method is simple, rapid, accurate, and highly sensitive.
建立了一种采用正己烷/乙醚/乙腈1:7:8 v/v/v混合溶剂萃取、快速、简便、廉价、有效、耐用且安全的净化及内标法定量气相色谱-质谱联用的19种邻苯二甲酸酯类化合物检测方法。该方法能有效去除乳制品中的脂质、脂肪酸和色素等干扰物质。19种邻苯二甲酸酯类化合物在0.025 - 0.2 mg/kg范围内，回收率为65.2 - 125.7%，相对标准偏差为7.9 - 15.4%（n = 6），检出限为0.005 - 0.02 mg/kg。10种乳制品原料和20种乳制品中19种邻苯二甲酸酯类化合物的浓度在0.01至0.12 mg/kg之间。所建立的方法简单、快速、准确且灵敏度高。
