Hirata M, Chang C H, Murad F
Department of Medicine, Stanford University School of Medicine, CA.
Biochim Biophys Acta. 1989 Mar 6;1010(3):346-51. doi: 10.1016/0167-4889(89)90060-8.
The effects of atrial natriuretic factor (ANF) on phosphoinositide hydrolysis were examined in preparations of cultured bovine aortic smooth muscle cells. In homogenates or particulate fractions from cultured bovine aortic smooth muscle cells, ANF and atriopeptin I increased the formation of inositol phosphates and GTPase activity. The effects on inositol phosphates were markedly enhanced with guanosine 5'[gamma-thio]triphosphate. Both atrial peptides also stimulated the formation of diacylglycerol in intact cultured cells. In these experiments, atriopeptin I was about 10-fold more potent than ANF. These studies indicate that atrial peptides have stimulatory effects on phosphoinositide hydrolysis which are mediated through a guanine nucleotide regulatory protein. The greater potency of atriopeptin I on GTPase activity and the accumulation of inositol phosphates suggests that the nonguanylate cyclase-coupled receptor for ANF (ANF-R2) mediates the stimulatory effects of ANF on phosphoinositide hydrolysis through a guanine nucleotide regulatory protein.
在培养的牛主动脉平滑肌细胞制剂中研究了心房利钠因子（ANF）对磷酸肌醇水解的影响。在培养的牛主动脉平滑肌细胞的匀浆或微粒部分中，ANF和心房肽I增加了肌醇磷酸的形成和GTP酶活性。用鸟苷5'-[γ-硫代]三磷酸可显著增强对肌醇磷酸的影响。两种心房肽也刺激完整培养细胞中甘油二酯的形成。在这些实验中，心房肽I的效力比ANF强约10倍。这些研究表明，心房肽对磷酸肌醇水解具有刺激作用，这是通过鸟嘌呤核苷酸调节蛋白介导的。心房肽I对GTP酶活性和肌醇磷酸积累的更强效力表明，ANF的非鸟苷酸环化酶偶联受体（ANF-R2）通过鸟嘌呤核苷酸调节蛋白介导ANF对磷酸肌醇水解的刺激作用。
