Anand-Srivastava M B, Cantin M
Biochem Biophys Res Commun. 1986 Jul 16;138(1):427-36. doi: 10.1016/0006-291x(86)90299-8.
We have studied the effect of synthetic rat atrial natriuretic factor (ANF) on adenylate cyclase activity in cultured cardiocytes from atria (left and right) and ventricles from neonatal rats. ANF (Arg 101-Tyr 126) inhibited adenylate cyclase activity in a concentration dependent manner in cultured atrial (right and left atria) and ventricular cells. However the inhibition was greater in atrial cells as compared to ventricular cells. The maximal inhibition observed in ventricular cells was about 35% with an apparent Ki of about 10(-10) M, whereas about 55% inhibition with an apparent Ki between 5 X 10(-10) M and 65% inhibition with an apparent Ki of 10(-9) M were observed in right and left atrial cardiocytes respectively. The inhibitory effect of ANF was dependent on the presence of guanine nucleotides. Various hormones and agents such as isoproterenol, prostaglandins, adenosine, forskolin and sodium fluoride stimulated adenylate cyclase activities to various degrees in these atrial and ventricular cardiocytes. ANF inhibited the stimulatory responses of all these agonists, however the degree of inhibition varied for each agent. In addition ANF also inhibited cAMP levels in these cells. These data indicate that ANF receptors are present in cardiocytes and are negatively coupled to adenylate cyclase.
我们研究了人工合成的大鼠心房利钠因子（ANF）对新生大鼠心房（左右心房）和心室培养心肌细胞中腺苷酸环化酶活性的影响。ANF（精氨酸101 - 酪氨酸126）在培养的心房（右心房和左心房）和心室细胞中以浓度依赖的方式抑制腺苷酸环化酶活性。然而，与心室细胞相比，心房细胞中的抑制作用更强。在心室细胞中观察到的最大抑制率约为35%，表观解离常数（Ki）约为10^(-10) M，而在右心房和左心房心肌细胞中分别观察到约55%的抑制率（表观Ki在5×10^(-10) M之间）和65%的抑制率（表观Ki为10^(-9) M）。ANF的抑制作用依赖于鸟嘌呤核苷酸的存在。各种激素和试剂如异丙肾上腺素、前列腺素、腺苷、福斯高林和氟化钠在这些心房和心室心肌细胞中不同程度地刺激腺苷酸环化酶活性。ANF抑制所有这些激动剂的刺激反应，然而每种试剂的抑制程度有所不同。此外，ANF还抑制这些细胞中的环磷酸腺苷（cAMP）水平。这些数据表明心肌细胞中存在ANF受体，并且这些受体与腺苷酸环化酶负偶联。
