Gillespie J I, Giraldez F, Greenwell J R
Department of Physiological Sciences, Medical School, The University, Newcastle upon Tyne, England.
FEBS Lett. 1989 Jan 16;243(1):17-20. doi: 10.1016/0014-5793(89)81208-6.
The effect of the mitogen bombesin on pHi in cells of the chick otic vesicle was studied using dual wavelength micro-spectrofluorimetric techniques. The results show that the bombesin did not produce an immediate alkalinization in quiescent otic vesicle cells nor a long-term change in pHi in vesicles reactivated to grow in culture. The recovery of pHi from an acid load, caused by an NH4 pulse, involved both Na+/H+ exchange and Na+:HCO3 influx mechanisms, neither of which was influenced by bombesin. These observations do not fit with a general model whereby pHi is a universal signal for DNA replication and cell proliferation.
采用双波长显微分光荧光技术研究了促有丝分裂剂蛙皮素对鸡耳泡细胞内pH值（pHi）的影响。结果表明，蛙皮素在静止的耳泡细胞中不会立即引起碱化，在重新激活以在培养中生长的耳泡中也不会引起pHi的长期变化。由NH4脉冲引起的酸负荷后pHi的恢复涉及Na+/H+交换和Na+:HCO3内流机制，这两种机制均不受蛙皮素的影响。这些观察结果与pHi是DNA复制和细胞增殖的通用信号这一普遍模型不符。
