Varela-Nieto I, Represa J, Avila M A, Miner C, Mato J M, Giraldez F
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Dev Biol. 1991 Feb;143(2):432-5. doi: 10.1016/0012-1606(91)90095-k.
The ability of an inositol phospho-oligosaccharide (POS) to mimic the mitogenic effects of nerve growth factor (NGF) and insulin on the early development of the inner ear was investigated. POS (10 microM) stimulated the incorporation of [3H]thymidine into the cochleovestibular ganglion by 3.9-fold. NGF (50 ng/ml) stimulation was 4.7-fold. POS and NGF showed no additivity. Cells induced to proliferate by POS overlapped with those expressing NGF receptors. POS, like insulin, potentiated the mitogenic effect of bombesin on the otic vesicle epithelium. DNA synthesis in the presence of bombesin (100 nM) plus POS (10 microM) was increased by 6.4-fold. POS stimulation was not additive with insulin. The results suggest that POS may play a role in growth factor regulation of cell proliferation during embryonic development.
研究了肌醇磷酸寡糖（POS）模拟神经生长因子（NGF）和胰岛素对内耳早期发育的促有丝分裂作用的能力。POS（10微摩尔）使[3H]胸苷掺入耳蜗前庭神经节的量增加了3.9倍。NGF（50纳克/毫升）刺激为4.7倍。POS和NGF没有相加作用。由POS诱导增殖的细胞与表达NGF受体的细胞重叠。POS与胰岛素一样，增强了蛙皮素对耳泡上皮的促有丝分裂作用。在存在蛙皮素（100纳摩尔）加POS（10微摩尔）的情况下，DNA合成增加了6.4倍。POS刺激与胰岛素没有相加作用。结果表明，POS可能在胚胎发育过程中细胞增殖的生长因子调节中发挥作用。
