Harenberg J, Giese C, Knödler A, Zimmermann R
1st Department of Medicine, Klinikum Mannheim, University of Heidelberg, FRG.
Haemostasis. 1989;19(1):13-20. doi: 10.1159/000215883.
The effects of a normal and a low molecular weight (LMW) heparin fraction were compared by four coagulation methods. Plasma samples of patients were investigated who were treated with normal heparin or with a LMW heparin. The study was undertaken to analyze the interrelationship between the coagulation methods: Heptest, activated partial thromboplastin time, thrombin clotting time, and S 2222 chromogenic anti-factor Xa test. The results showed a high correlation between Heptest and S 2222 anti-factor Xa method for unfractionated and LMW heparin (r = 0.91 and 0.90). Comparing the coagulation times in seconds of Heptest and aPTT, the correlations were r = 0.56 (normal heparin) and 0.15 (LMW heparin). The correlation between the coagulation times of Heptest and thrombin clotting time were r = 0.65 and 0.25, respectively. The correlations between the coagulation methods were higher, when coagulation times rather than transformed values to units per liter of the Heptest and of the S 2222 anti-factor Xa method were employed. Furthermore, the data demonstrate a high sensitivity of the Heptest to conventional and LMW heparin, whereas activated partial thromboplastin time and thrombin clotting time are less sensitive to either heparin. For laboratory control of LMW heparin, Heptest and S 2222 chromogenic method are reliable tests. Therapeutic ranges will have to be established.
通过四种凝血方法比较了普通肝素和低分子量（LMW）肝素组分的效果。对接受普通肝素或LMW肝素治疗的患者血浆样本进行了研究。开展该研究以分析凝血方法之间的相互关系：Heptest法、活化部分凝血活酶时间、凝血酶凝血时间和S 2222生色抗Xa因子试验。结果显示，对于普通肝素和LMW肝素，Heptest法与S 2222抗Xa因子法之间具有高度相关性（r = 0.91和0.90）。比较Heptest法和活化部分凝血活酶时间以秒为单位的凝血时间，相关性分别为r = 0.56（普通肝素）和0.15（LMW肝素）。Heptest法与凝血酶凝血时间的凝血时间之间的相关性分别为r = 0.65和0.25。当采用凝血时间而非将Heptest法和S 2222抗Xa因子法换算为每升单位的换算值时，凝血方法之间的相关性更高。此外，数据表明Heptest法对普通肝素和LMW肝素具有高敏感性，而活化部分凝血活酶时间和凝血酶凝血时间对任一种肝素的敏感性较低。对于LMW肝素的实验室监测，Heptest法和S 2222生色法是可靠的检测方法。必须确定治疗范围。
