Xian Zhiqiang, Huang Wei, Yang Yingwu, Tang Ning, Zhang Chao, Ren Maozhi, Li Zhengguo
Genetic Engineering Research Centre, School of Life Sciences, Chongqing University, Chongqing 400044, People's Republic of China.
Genetic Engineering Research Centre, School of Life Sciences, Chongqing University, Chongqing 400044, People's Republic of China
J Exp Bot. 2014 Dec;65(22):6655-66. doi: 10.1093/jxb/eru387. Epub 2014 Nov 5.
In Arabidopsis thaliana, Argonaute1 (AGO1) interacts with miR168 to modulate the small RNA regulatory pathway. However, the underlying mechanism of regulation and relationship between AGO1 and miR168 is poorly understood in the cash crop Solanum lycopersicum (tomato). We previously found that SlAGO1A and SlAGO1B were cleaved by miR168 in tomato. In this study, we show that SlAGO1A and SlAGO1B accumulate in miR168-sponge transgenic plants, and that expression of miR168-resistant SlAGO1A (4m-SlAGO1A) and SlAGO1B (4m-SlAGO1B) in tomato results in a series of defects affecting growth rate, floral timing, leaves, and fruit. Accumulation of miR156 was found when 4m-SlAGO1A was at an early developmental stage compared to the wild type and original SlAGO1A transgenic plants, and miR172 was highly expressed in adult 4m-SlAGO1A compared to the controls. In addition, the expression of multiple small RNAs was altered in 4m-SlAGO1A. Taken together, our data provide novel insights into the interaction between SlAGO1s and miR168 in determining growth rate, phase change, leaf epinasty, fruit initiation and expansion, and other developmental processes in tomato.
在拟南芥中，AGO1（AGO1）与miR168相互作用以调节小RNA调控途径。然而，在经济作物番茄中，AGO1与miR168之间潜在的调控机制及其关系仍知之甚少。我们之前发现番茄中的SlAGO1A和SlAGO1B被miR168切割。在本研究中，我们发现SlAGO1A和SlAGO1B在miR168海绵转基因植物中积累，并且在番茄中表达抗miR168的SlAGO1A（4m-SlAGO1A）和SlAGO1B（4m-SlAGO1B）会导致一系列影响生长速率、花期、叶片和果实的缺陷。与野生型和原始SlAGO1A转基因植物相比，在4m-SlAGO1A处于早期发育阶段时发现miR156积累，而与对照相比，miR172在成年4m-SlAGO1A中高表达。此外，4m-SlAGO1A中多种小RNA的表达发生了改变。综上所述，我们的数据为SlAGO1s与miR168在决定番茄生长速率、阶段变化、叶片内卷、果实起始和膨大以及其他发育过程中的相互作用提供了新的见解。
