Shafren D R, Tannock G A, Roberts T K
Department of Biological Sciences, University of Newcastle, New South Wales, Australia.
Res Vet Sci. 1989 Jan;46(1):95-9.
A rapid sensitive enzyme-linked immunosorbent assay for the detection of antibody to avian encephalomyelitis viruses (AEVs) in chickens using purified antigen is described. The procedure differed from others which have been described for AEV, in that it involved a negative antigen subtraction step which accounted for the variable adhesiveness of chicken sera to plastic surfaces. The procedure was reproducible (between-assay coefficient of variation 8.95 per cent) and a good correlation was observed with results obtained by neutralisation index tests (r = 0.91, P less than 0.1). The assay detects only AEV-specific antibody and allows monitoring of the spread of AEV in flocks.
描述了一种使用纯化抗原快速灵敏地检测鸡群中禽脑脊髓炎病毒（AEV）抗体的酶联免疫吸附测定法。该方法与已报道的其他检测AEV的方法不同，它包含一个阴性抗原扣除步骤，该步骤考虑了鸡血清对塑料表面的可变粘附性。该方法具有可重复性（批间变异系数为8.95%），并且与通过中和指数试验获得的结果具有良好的相关性（r = 0.91，P小于0.1）。该测定法仅检测AEV特异性抗体，并可监测AEV在鸡群中的传播情况。
